생물학적 소스
goat
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
secondary antibodies
클론
polyclonal
형태
buffered aqueous solution
종 반응성
rabbit
농도
~1 mg/mL
형광
λex 635 nm; λem 655 nm in PBS, pH 7.4
저장 온도
−20°C
일반 설명
Abberior STAR 635 is a dye developed for STED and confocal imaging in the red spectral region. It features exceptional photostability, very high brightness and low background labeling. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR 635 can substitute dyes like Atto™ 647N, Alexa Fluor™ 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 635 (PBS pH 7.4), 635 (H2O), 635 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 110 000 (PBS pH 7.4), 110 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,26 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,38 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 656 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,88 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 2,8 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 635 (PBS pH 7.4), 635 (H2O), 635 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 110 000 (PBS pH 7.4), 110 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,26 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,38 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 656 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,88 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 2,8 (PBS pH 7.4)
애플리케이션
Abberior STAR 635 particularly well suits the Abberior Instruments 2-color 775nm STED microscope and the Leica TCS STED system. It delivers high-resolution STED images with very low background signal due to its improved water solubility and exceptional brightness. The dye serves as an ideal partner for several 2-color STED packages.
적합성
Designed and tested for fluorescent super-resolution microscopy
분석 메모
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
법적 정보
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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Development (Cambridge, England), 139(20), 3775-3785 (2012-09-20)
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Endosomal Sorting Complex Required for Transport (ESCRT) proteins can be transiently recruited to the plasma membrane for membrane repair and formation of extracellular vesicles. Here, we discovered micrometer-sized worm-shaped ESCRT structures that stably persist for multiple hours at the plasma
Novel red fluorophores with superior performance in STED microscopy.
Wurm, C.A., et al.
Optical Nanoscopy, 1, 7-7 (2012)
Tobias Müller et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 13(8), 1986-2000 (2012-03-01)
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