생물학적 소스
goat
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
secondary antibodies
클론
polyclonal
형태
buffered aqueous solution
종 반응성
rabbit
농도
~1 mg/mL
형광
λex 511 nm; λem 530 nm in PBS, pH 7.4
저장 온도
−20°C
일반 설명
Abberior STAR 512 was developed for STED and confocal microscopy in the green spectral region. Abberior STAR 512 is highly photostable and bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation lasers at 488 nm or 514 nm. Abberior STAR 512 can substitute dyes like Oregon Green 514, Atto™ 514 or Alexa Fluor™ 514. For STED microscopy, Abberior STAR 512 can be most efficiently used with STED Laser wavelengths between 590 - 610 nm. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (PBS pH 7.4), 512 (H2O), 517 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 92 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,24 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,07 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 530 (PBS pH 7.4), 530 (H2O), 533 (MeOH)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,82 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (PBS pH 7.4), 512 (H2O), 517 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 92 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,24 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,07 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 530 (PBS pH 7.4), 530 (H2O), 533 (MeOH)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,82 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (PBS pH 7.4)
적합성
Designed and tested for fluorescent super-resolution microscopy
분석 메모
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
법적 정보
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
S. M. Polyakova, et al.,
European Journal of Organic Chemistry, 30, 5162-5177 (2009)
Gyuzel Yu Mitronova et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 16(15), 4477-4488 (2010-03-24)
New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N',N-bis(2,2,2-trifluoroethyl) derivatives 1 e, 1 i, 1 j, 3-H and 5 were found to
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