W0625

Wright stain

≤4% purity (HPLC), powder

• 동의어(들): Eosin Methylene blue according to Wright
• CAS Number: 68988-92-1
• MDL number: MFCD00082143
• UNSPSC 코드: 12171500
• NACRES: NA.47

속성

• product name: Wright stain, suitable for blood stain
• 분석: ≤4% (HPLC)
• 형태: powder
• 농도: ≤4% (HPLC)
• 색상: dark green
• solubility: methanol: 10 mg/10 mL
• 적합성: suitable for blood stain
• 응용 분야: diagnostic assay manufacturing; hematology; histology
• 저장 온도: room temp
• InChI: 1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1
• InChI key: AXIKDPDWFVPGOD-UHFFFAOYSA-O

설명

일반 설명

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

애플리케이션

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

• It is also used for G-banding and helps in the analysis of poorly spread metaphases.
• It has been used to study the cytogenetics of human hematopoietic cells.
• It has also been employed in the karyotyping of human embryonic stem cells.
• It is also used in the staining of chromosomes.
• It is used for the exudate analysis of white blood cell concentration.

Reported to be superior to May-Grunwald Stain in blood staining.

특징 및 장점

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

제조 메모

Prepared using polychromed (oxidized) methylene blue and eosin Y.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302,H319
• 예방조치 성명서: P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves