콘텐츠로 건너뛰기
Merck
모든 사진(1)

주요 문서

83205

Sigma-Aldrich

Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae)

powder, off-white, ~70 U/mg

동의어(들):

Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

로그인조직 및 계약 가격 보기


About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204

양식

powder

특이 활성도

~70 U/mg

분자량

Mr ~71000

색상

off-white

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.

애플리케이션

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.

단위 정의

1 U corresponds to the amount of enzyme which will liberate 1 μmol of phosphate from inorganic pyrophosphate per minute at pH 7.2 and 25 °C

기타 정보

Mechanism of action

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

도움이 필요하시면 연락하세요. 고객 지원 부서

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

The mechanism of action of yeast inorganic pyrophosphatase.
B S Cooperman
Methods in enzymology, 87, 526-548 (1982-01-01)
Manhong Yao et al.
Molecular biology reports, 39(8), 7989-7996 (2012-04-28)
According to sequences of H(+)-pyrophosphatase genes from GenBank, a new H(+)-pyrophosphatase gene (KfVP1) from the halophyte Kalidium foliatum, a very salt-tolerant shrub that is highly succulent, was obtained by using reverse transcription PCR and rapid amplification of cDNA ends methods.
Genetic manipulation of a "vacuolar" H(+)-PPase: from salt tolerance to yield enhancement under phosphorus-deficient soils.
Roberto A Gaxiola et al.
Plant physiology, 159(1), 3-11 (2012-03-22)
Habib Khoudi et al.
Journal of plant physiology, 169(1), 98-103 (2011-11-08)
Cadmium (Cd) is considered an extremely significant pollutant due to its high toxicity to many organisms. Plants have evolved several mechanisms to cope with Cd, the most important of which is vacuolar sequestration. Cadmium can be directly transported into vacuoles
Zh I Rybchenko et al.
Ukrains'kyi biokhimichnyi zhurnal (1999 ), 84(3), 88-94 (2012-08-07)
The function of two electrogenic H+ -pumps in plant vacuolar membrane (tonoplast) and their response to the salt stress conditions and adaptogenic preparations have been studied. Experiments were carried out on tonoplast fraction isolated from the roots of corn seedlings

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.