추천 제품
양식
powder
특이 활성도
~70 U/mg
분자량
Mr ~71000
색상
off-white
저장 온도
−20°C
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일반 설명
Molecular Weight: 71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.
Extinction Coefficient: E1% = 14.5 (280 nm)
pI: 4.75
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.
애플리케이션
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.
It plays an important role in protein, RNA, and DNA synthesis.
단위 정의
1 U corresponds to the amount of enzyme which will liberate 1 μmol of phosphate from inorganic pyrophosphate per minute at pH 7.2 and 25 °C
기타 정보
Mechanism of action
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
가장 최신 버전 중 하나를 선택하세요:
The mechanism of action of yeast inorganic pyrophosphatase.
B S Cooperman
Methods in enzymology, 87, 526-548 (1982-01-01)
Manhong Yao et al.
Molecular biology reports, 39(8), 7989-7996 (2012-04-28)
According to sequences of H(+)-pyrophosphatase genes from GenBank, a new H(+)-pyrophosphatase gene (KfVP1) from the halophyte Kalidium foliatum, a very salt-tolerant shrub that is highly succulent, was obtained by using reverse transcription PCR and rapid amplification of cDNA ends methods.
Genetic manipulation of a "vacuolar" H(+)-PPase: from salt tolerance to yield enhancement under phosphorus-deficient soils.
Roberto A Gaxiola et al.
Plant physiology, 159(1), 3-11 (2012-03-22)
Habib Khoudi et al.
Journal of plant physiology, 169(1), 98-103 (2011-11-08)
Cadmium (Cd) is considered an extremely significant pollutant due to its high toxicity to many organisms. Plants have evolved several mechanisms to cope with Cd, the most important of which is vacuolar sequestration. Cadmium can be directly transported into vacuoles
Zh I Rybchenko et al.
Ukrains'kyi biokhimichnyi zhurnal (1999 ), 84(3), 88-94 (2012-08-07)
The function of two electrogenic H+ -pumps in plant vacuolar membrane (tonoplast) and their response to the salt stress conditions and adaptogenic preparations have been studied. Experiments were carried out on tonoplast fraction isolated from the roots of corn seedlings
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