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Merck
모든 사진(3)

문서

48722

Millipore

Gelatin from porcine skin

medium gel strength, suitable for microbiology

동의어(들):

Gelatine, hydrolyzed collagen, collagen hydrolysate, gelatine hydrolysate

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
41106212
NACRES:
NA.85

생물학적 소스

Porcine

Quality Level

무균

non-sterile

형태

powder

품질

medium gel strength

포장

bottle of 100 g
bottle of 500 g

무기 잔류물

≤2%

손실

≤15% loss on drying

색상

light yellow

pH

4.0-6.0 (25 °C, 67 mg/mL in H2O)

겔 강도

165-195 g Bloom (67 mg/ml water)

solubility

H2O: 67 mg/mL at 50 °C, slightly hazy, slightly yellow

음이온 미량물

chloride (Cl-): ≤3000 mg/kg

양이온 미량물

Ca: ≤2000 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤10 mg/kg
Cu: ≤50 mg/kg
Fe: ≤50 mg/kg
K: ≤500 mg/kg
Mg: ≤500 mg/kg
Mn: ≤5 mg/kg
Na: ≤5000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤10 mg/kg

응용 분야

microbiology

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred to as type A. It is a hydrocolloid and is rich in glycine, proline, and hydroxyproline, which impart structural stability. It is synthesized from the alkaline digestion of collagen from porcine. Gelatin takes up a random coil structure after digestion from the triple helical collagen.

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc., in water. Type A gelatin is derived from acid-cured tissue, and Type B from lime-cured tissue.

The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 180.

애플리케이션

Gelatin has been used in many applications, such as in coating cell culture plates to improve attachment of cells, in PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology.

성분

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

주의사항

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

제조 메모

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. Manufactured by Gelita AG

Storage Class Code

11 - Combustible Solids

WGK

nwg

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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