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주요 문서

COO/COA

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39145

N-(7-Dimethylamino-4-methyl-3-coumarinyl)maleimide

Name: <I>N</I>-(7-Dimethylamino-4-methyl-3-coumarinyl)maleimide
Brand: SIAL

for fluorescence, ≥99.0% (HPLC)

동의어(들):

DACM-3

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About This Item

실험식(Hill 표기법):

C₁₆H₁₄N₂O₄

CAS Number:

55145-14-7

Molecular Weight:

298.29

Beilstein:

1545346

EC Number:

259-499-0

MDL number:

MFCD00006863

UNSPSC 코드:

12352200

PubChem Substance ID:

329755824

Grade

for fluorescence

분석

≥99.0% (HPLC)

mp

218-220 °C (lit.)

형광

λ_ex 398 nm; λ_em 482 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol)

적합성

suitable for fluorescence

SMILES string

CN(C)c1ccc2C(C)=C(N3C(=O)C=CC3=O)C(=O)Oc2c1

InChI

1S/C16H14N2O4/c1-9-11-5-4-10(17(2)3)8-12(11)22-16(21)15(9)18-13(19)6-7-14(18)20/h4-8H,1-3H3

InChI key

ADEORFBTPGKHRP-UHFFFAOYSA-N

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기타 정보

Fluorescent reagent with high selectivity for protein thiols and with superior fluorescence and water solubility properties than dansyl chloride

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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Y. Kanaoka

Angewandte Chemie (International Edition in English), 89, 141-141 (1977)

Changes in SH reactivity of the protein in porcine intestinal brush-border membranes associated with lipid peroxidation.

T Ohyashiki et al.

Journal of biochemistry, 115(2), 224-229 (1994-02-01)

The effects of lipid peroxidation on the SH reactivity of the proteins in porcine intestinal brush-border membranes were examined using a fluorogenic thiol reagent, N-[7-dimethyl-amino-4-methylcoumarinyl]maleimide (DACM) in relation to lipid organization. Changes in the lipid organization were assessed by measurement

Trypanosoma cruzi phospho enol pyruvate carboxykinase (ATP-dependent): transition metal ion requirement for activity and sulfhydryl group reactivity.

L A Jurado et al.

Biochimica et biophysica acta, 1292(1), 188-196 (1996-01-04)

We studied the transition metal ion requirements for activity and sulfhydryl group reactivity in phospho enol pyruvate carboxykinase (PEP-carboxykinase; ATP:oxaloacetate carboxylase (transphosphorylating), EC 4.1.1.49), a key enzyme in the energy metabolism of the protozan parasite Trypanosoma (Schizotrypanum) cruzi. As for

A sensitive enzymatic method for paraoxon detection based on enzyme inhibition and fluorescence quenching.

Kuikui Wang et al.

Talanta, 84(2), 400-405 (2011-03-08)

A sensitive and selective method for the paraoxon detection based on enzyme inhibition and fluorescence quenching was presented in this study. Under the catalytic effect of acetylcholinesterase (AChE), acetylthiocholine (ATCh) hydrolysis released thiocholine (TCh) which could react with N-(7-dimethylamino-4-methylcoumarin-3-yl) maleimide

The functions of the ninth component of human complement are sustained by disulfide bonds with different susceptibilities to reduction.

M Hatanaka et al.

Biochimica et biophysica acta, 1209(1), 117-122 (1994-11-16)

Purified C9 with expected hemolytic and polymerizing activities was found to contain approximately 0.2 mol of sulfhydryl groups/mol of C9. By proteolysis of C9 with labeled SH groups, the SH residues on intact C9 were mapped to Cys-359 and Cys-384

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