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Merck
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문서

DNTPM-RO

Roche

PCR Nucleotide Mix

solution, sufficient for 500 reactions (11581295001), sufficient for 5,000 reactions (11814362001), sufficient for 2,500 reactions (04638956001)

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About This Item

UNSPSC 코드:
41106300
NACRES:
NA.21

Grade

Molecular Biology

Quality Level

100

분석

99.8% (sum of 4 peaks, HPLC)

형태

solution

사용

sufficient for 2,500 reactions (04638956001)
 sufficient for 5,000 reactions (11814362001)
 sufficient for 500 reactions (11581295001)

포장

pkg of 10 × 200 μL (11814362001 [10mM, each])
pkg of 200 μL (11581295001 [10 mM, each])
pkg of 5 × 200 μL (04638956001 [10 mM, each])

제조업체/상표

Roche

농도

10 mM

불순물

DNase, none detected
 RNase, none detected

색상

colorless

solubility

water: miscible

저장 온도

−20°C

관련 카테고리

일반 설명

PCR Nucleotide Mix is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, and dTTP, each at a concentration of 10 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.

애플리케이션

  • The PCR Nucleotide Mix is optimized for use in all types of amplification reactions and primer-extension reactions: PCR
  • RT-PCR
  • cDNA synthesis
  • Primer extension

특징 및 장점

The PCR Nucleotide Mix can be added directly to amplification reactions. PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity.

Improve yield and performance
  • Choose a convenient ready-to-use mix of all 4 nucleotides.
  • Achieve the highest PCR and RT-PCR sensitivity.
  • Insist on highest purity (>99%) nucleotides.

포장

1 set containing 4 ready-to-use mixes

품질

Function Testing in PCR
Function Testing in RT-PCR
Absence of Contaminating Deoxyribonucleases/Nicking Activities according to the current Quality Control procedures.
Absence of Contaminating Ribonucleases according to the current Quality Control procedures.

제조 메모

Activator: Mg2+
Working solution: If required, the solutions can be diluted with distilled autoclaved water.
Storage conditions (working solution): It will withstand 50 freeze/thaw cycles.

기타 정보

For life science research only. Not for use in diagnostic procedures.

키트 구성품 전용

제품 번호
설명
  • dATP, PCR Grade, sodium salt 10 mM
  • dCTP, PCR Grade, sodium salt 10 mM
  • dGTP, PCR Grade, sodium salt 10 mM
  • dTTP, PCR Grade, sodium salt 10 mM

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Weimin Liu et al.
Protocol exchange, 2010 (2010-09-28)
Conventional PCR followed by molecular cloning and sequencing of amplified products is commonly used to test clinical specimens for target sequences of interest, such as viral, bacterial or parasite nucleic acids. However, this approach has serious limitations when used to
Tania Nolan et al.
Nature protocols, 1(3), 1559-1582 (2007-04-05)
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. Although it is often described

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