11371517001

Swa I

from Staphylococcus warneri

• UNSPSC 코드: 12352204

속성

• 양식: solution
• 포장: pkg of 200 U (10 U/μl)
• 제조업체/상표: Roche
• 파라미터: 25 °C optimum reaction temp.
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

특이성

Recognition sites: ATTTAAAT
ATTTAAAT
Restriction site: ATTT↓AAAT
ATTT↓AAAT
Heat inactivation: Swa I can be heat inactivated by incubation at 65 °C for 20 minutes.

애플리케이션

Swa I is very useful for digesting GC-rich bacterial genomes.

DNA 프로파일

Number of cleavage sites on different DNAs

• λ: 0
• φX174: 0
• Ad2: 1
• M13mp7: 1
• pBR322: 0
• pBR328: 0
• SV40: 1

단위 정의

One unit is the enzyme activity that completely cleaves 1 μg Ad2 × Ssp I DNA in one hour at +25 °C in a total volume of 25 μl (1x) SuRE/Cut buffer H.

분석 메모

SuRE/Cut Buffer System
The buffer in bold is recommended for optimal activity

• A: 0-10%
• B: 10-25%
• H: 100%
• L: 0-10%
• M: 0-10%

Activity in PCR buffer: Not tested

기타 정보

For life science research only. Not for use in diagnostic procedures.

키트 구성품 전용

• Enzyme Solution
• SuRE/Cut Buffer H 10x concentrated

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash