추천 제품
형태
solid
Quality Level
사용
sufficient for ~50 tests
분자량
105.99 g/mol
제조업체/상표
Roche
기술
activity assay: suitable
색상
white to light yellow-brown
solubility
water: soluble ~210 g/L at 68 °F (Stop reagent)
soluble ~71 g/L at 32 °F (stop reagent)
water: soluble (Buffer substance, lyophilized substrate)
적합성
suitable for enzyme test
응용 분야
life science and biopharma
저장 온도
room temp
일반 설명
N-acetyl-β-D-glucosaminidase (NAG) is mainly used in the colorimetric assay for the determination of N-acetyl-β-D-glucosaminidase in urine. NAG hydrolyzes 3-cresolsulfonphthaleinyl-N-acetyl-D-glucosaminide to release 3-cresolsulfonphthalein, which is measured photometrically at 580nm. It is an intracellular lysosomal enzyme, present in the S3 segment of proximal tubular cells and distal nephron. Presence of NAG in the urine shows an organelle impairment in proximal tubule. Level of NAG rises in individuals over 70 years of age.
애플리케이션
N-acetyl-β-D-glucosaminidase (NAG) has been used for preparing standards for the determination of N-acetyl-β-D-glucosaminidase in urine in life-science research applications.
포장
1 kit containing 3 components
제조 메모
Working solution: Preparation of solutions:
Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 °C, protected from light.
Solution III is stable for 1 month stored at 2 to 8 °C.
Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 °C and for one month when stored at -15 to -25 °C.
- Buffer solution
Dissolve the contents of bottle 1 with 55 ml double-distilledwater. - Substrate solution
Dissolve the contents of bottle 2 with 55 ml solution I. - Stop reagent
Dissolve the contents of bottle 3 with 110 ml double-distilled water.
Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 °C, protected from light.
Solution III is stable for 1 month stored at 2 to 8 °C.
Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 °C and for one month when stored at -15 to -25 °C.
저장 및 안정성
Keep container tightly closed in a dry and well-ventilated place.
기타 정보
For life science research only. Not for use in diagnostic procedures.
키트 구성품 전용
제품 번호
설명
- Buffer Substance consisting of citric acid and potassium citrate
- Lyophilized Substrate consisting of sodium 3-cresolsulfonphthaleinyl-N-acetyl-β-D-glucosaminide and borax
- Stop Reagent consisting of sodium carbonate
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Anne-Sophie Truche et al.
Transplantation proceedings, 51(10), 3234-3243 (2019-11-17)
This study aimed to identify transplantation characteristics and biomarkers that predict outcomes for kidney transplant (KT) patients from donors after circulatory death (DCDs). Consecutive patients receiving a KT from a DCD in our center between 2014 and 2016 were included;
Clinical Nephrotoxins: Renal Injury from Drugs and Chemicals (2008)
Julia M Hofstra et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 23(8), 2546-2551 (2008-03-01)
An accurate prediction of prognosis in patients with idiopathic membranous nephropathy (iMN) would allow restriction of immunosuppressive treatment to patients who are at highest risk for end-stage renal disease (ESRD). Several markers of proximal tubular cell injury have been used
Maha Al-Asmakh et al.
Frontiers in pharmacology, 11, 569402-569402 (2021-02-26)
Chronic kidney disease (CKD) may be fatal for its victims and is an important long-term public health problem. The complicated medical procedures and diet restrictions to which patients with CKD are subjected alter the gut microbiome in an adverse manner
Raphael Allgaier et al.
Nephrology (Carlton, Vic.), 25(12), 888-896 (2020-08-26)
Chronic heart failure may lead to chronic kidney disease. Previous studies suggest tubular markers N-acetyl-b-D-glucosaminidase (NAG) and Kidney-injury-molecule-1 (KIM-1) as potential markers for the cardiorenal syndrome (CRS). The prognostic value of NAG and KIM-1 regarding implantable cardioverter defibrillator (ICD) shock
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.