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Merck
모든 사진(1)

문서

SCT036

Sigma-Aldrich

BioTracker 625 Red GSH Dye

Live cell imaging dye for glutathione (GSH) that detects reactive oxygen species (ROS) free radicals.

동의어(들):

Live cell imaging probe

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About This Item

UNSPSC 코드:
12352207
eCl@ss:
32161000
NACRES:
NA.47

기술

cell based assay: suitable

검출 방법

fluorometric

배송 상태

ambient

일반 설명

Glutathione (GSH) is an important antioxidant preventing damage to important cellular components caused by reactive oxygen species (ROS) including free radicals, peroxides, lipid peroxides, and heavy metals. Alterations in glutathione homeostasis are associated with a variety of diseases; therefore, real-time live-cell imaging and quantification of GSH dynamics are important for understanding pathophysiological processes.

The BioTracker 625 Red GSH Dye is probe for glutathione (GSH) in living cells. The dye is formed with an orange fluorescent dye (T) and a deep-red fluorescent dye (S). At a low GSH concentration, green light excitation of T, leads to an energy transfer to S and a subsequent emission of deep-red fluorescent light. At a high GSH concentration, the energy transfer does not occur, so the probe emits the orange fluorescent light from the emission of T. The dye produces a quick and reversible response to the GSH concentration changes, relatively slow photobleaching and low cytotoxicity.

Spectral Properties
Absorbance: 520nm
Emission: 625nm and 582nm

애플리케이션

Live cell fluorescent imaging
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

물리적 형태

Lyophilized

저장 및 안정성

Store BioTracker 625 Red GSH Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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