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Merck
모든 사진(1)

문서

PC234L

Sigma-Aldrich

Anti-Vasopressin Rabbit pAb

lyophilized, Calbiochem®

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.43

생물학적 소스

rabbit

Quality Level

항체 형태

serum

항체 생산 유형

primary antibodies

클론

polyclonal

형태

lyophilized

포함

≤0.1% sodium azide as preservative

종 반응성

rat

제조업체/상표

Calbiochem®

저장 조건

OK to freeze

동형

IgG

배송 상태

ambient

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

rat ... Avp(24221)

일반 설명

Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes vasopressin.
Recognizes vasopressin in rat hypothalamus.
This Anti-Vasopressin Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Vasopressin.

면역원

Rat
rat arginine vasopressin

애플리케이션


Frozen Sections (1:400-1:800, Cy3; 1:2000-1:4000, Bn-SA/HRP)
Immunofluorescence (1:200-1:400)

경고

Toxicity: Standard Handling (A)

물리적 형태

Undiluted serum.

재구성

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months. Avoid freeze/thaw cycles of solutions.

분석 메모

Positive Control
Rat hypothalamus

기타 정보

Antibody specificity was examined in the rat paraventricular nucleus and supraoptic nucleus. Staining is completely eliminated by pretreatment of antibody with arginine vasopressin (10 µg/ml). Preabsorption of antibody with oxytocin (100 µg/ml) resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
Sudhof, T.C. 1995. Nature375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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Yarely C Hoffiz et al.
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Long-standing clinical findings report a dramatic surge of vasopressin in umbilical cord blood of the human neonate, but the neural underpinnings and function(s) of this phenomenon remain obscure. We studied neural activation in perinatal mice and rats, and found that
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Acta physiologica (Oxford, England), 237(3), e13896-e13896 (2022-10-18)
Physiological functions in mammals show circadian oscillations, synchronized by daily cycles of light and temperature. Central and peripheral clocks participate in this regulation. Since the ion channel TRPM8 is a critical cold sensor, we investigated its role in circadian function.
V Tobin et al.
Journal of neuroendocrinology, 24(4), 629-641 (2011-10-13)
In magnocellular neurones of the supraoptic nucleus (SON), the neuropeptides vasopressin and oxytocin are synthesised and packaged into large dense-cored vesicles (LDCVs). These vesicles undergo regulated exocytosis from nerve terminals in the posterior pituitary gland and from somata/dendrites in the

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