추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
NM-11, monoclonal
형태
liquid
미포함
preservative
종 반응성
human, rat
반응하면 안 됨
mouse
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
avoid repeated freeze/thaw cycles
동형
IgG1
배송 상태
wet ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... EP300(2033)
일반 설명
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.
Recognizes the p300 protein in A431, HeLa, and ML-1 cells.
This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.
면역원
Epitope: within amino acids 2071-2091 near the CBP C-terminus
Human
recombinant, human p300 protein
애플리케이션
Frozen Sections (see application references)
Immunoblotting (1 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (1-5 µg/ml, heat pre-treatment required)
Immunoblotting (1 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (1-5 µg/ml, heat pre-treatment required)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
물리적 형태
In 0.05 M sodium phosphate buffer, 50% glycerol.
재구성
Following initial thaw, aliquot and freeze (-20°C).
분석 메모
Positive Control
A431, HeLa, or ML-1 cells
A431, HeLa, or ML-1 cells
기타 정보
Antibody should be titrated for optimal results in individual systems.
Dallas, P.B., et al. 1997. Hybridoma16, 273.
Shisler, J., et al. 1996. J. Virol.70, 68.
Wang, H.G., et al. 1995. J. Virol.69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene9, 1733.
Eckner, R., et al. 1994. Genes Dev.8, 869.
Abraham, S.E., et al. 1993. Oncogene8, 1639.
Wang, H.G., et al. 1993. J. Virol.67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol.11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol.11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol.8, 3955.
Yee, S. and Branton, P., 1985. Virology147, 142.
Shisler, J., et al. 1996. J. Virol.70, 68.
Wang, H.G., et al. 1995. J. Virol.69, 7917.
Banerjee, A.C.,et al. 1994. Oncogene9, 1733.
Eckner, R., et al. 1994. Genes Dev.8, 869.
Abraham, S.E., et al. 1993. Oncogene8, 1639.
Wang, H.G., et al. 1993. J. Virol.67, 476.
Howe, J.A., and Bayley, S.T., 1992. Virology186, 15.
Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol.11, 2826.
Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol.11, 5389.
Egan, C., et al. 1988. Mol. Cell. Biol.8, 3955.
Yee, S. and Branton, P., 1985. Virology147, 142.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Kevin M Elias et al.
JCI insight, 1(13) (2016-09-13)
Regulation of lineage-restricted transcription factors has been shown to influence malignant transformation in several types of cancer. Whether similar mechanisms are involved in ovarian cancer pathogenesis is unknown. PAX8 is a nuclear transcription factor that controls the embryologic development of
Daniel Stauffer et al.
The Journal of biological chemistry, 282(13), 9678-9687 (2007-02-03)
The highly related acetyltransferases, p300 and CREB-binding protein (CBP) are coactivators of signal-responsive transcriptional activation. In addition, recent evidence suggests that p300/CBP also interacts directly with complexes that mediate DNA replication and repair. In this report, we show that loss
Karthigayan Shanmugasundaram et al.
Nature communications, 8(1), 997-997 (2017-10-21)
The molecular mechanisms that couple glycolysis to cancer drug resistance remain unclear. Here we identify an ATP-binding motif within the NADPH oxidase isoform, NOX4, and show that ATP directly binds and negatively regulates NOX4 activity. We find that NOX4 localizes
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