MABT852M

Anti-Podoplanin Antibody, clone LpMab-21

clone LpMab-21, from mouse

• 동의어(들): Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: LpMab-21, monoclonal
• 종 반응성: human
• 포장: antibody small pack of 25 μg
• 기술: ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable (paraffin)
• 동형: IgG2aκ
• NCBI 수납 번호: NP_006465.3
• UniProt 수납 번호: Q86YL7
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PDPN(10630)

설명

일반 설명

Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. A high degree of expression is observed in placenta, lung, skeletal muscle, and brain. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. For clone LpMab-21 a minimum epitope is determined to be Thr76-Arg79 of the human podoplanin with sialic acid linked to Thr76. LpMab-21 39 detects human podoplanin expression in glioblastoma, oral squamous carcinoma, and seminoma 40 cells as well as in normal lymphatic endothelial cells. However, it does not react with renal glomerular epithelial cells or lung type I alveolar cells, indicating that sialylation of human podoplanin Thr76 is cell-type specific. (Ref.: Kato, Y et al (2017). Monoclon Antib Immunodiagn Immunother. 36(1); 20-24; Kaneko, MK et al. (2017). Cancer Med.; 6(2):382-396).

특이성

Clone LpMab-21 detects human podoplanin. It targets a minimum epitope Thr76-Arg79 witih sialic acid linked to Thr76.

면역원

LN229 glioma cells transfected with human podoplanin.

애플리케이션

Anti-Podoplanin, clone LpMab-21, Cat. No. MABT852, is a mouse monoclonal antibody that detects human Podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kaneko, M.K., et. al. (2017). Cancer Med. 6(2):382-396).

ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kaneko, M.K., et. al. (2017). Cancer Med. 6(2):382-396).

Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kaneko, M.K., et. al. (2017). Cancer Med. 6(2):382-396).

Research Category
Cell Structure

품질

Evaluated by Flow Cytometry in HEK293 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Podoplanin in one million HEK293 cells.

표적 설명

16.70 kDa calculated.

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1