MABT819

Anti-Glycosaminoglycan Antibody, skin specific Antibody, clone PG-4

clone PG-4, from mouse

• 동의어(들): Proteoglycan, Dermatan sulfate, Chondroitin sulfate
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: PG-4, monoclonal
• 종 반응성: human, chicken, shark
• 포장: antibody small pack of 25 μL
• 기술: ELISA: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgMκ
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FAM20B(9917)

설명

일반 설명

Proteoglycans (PG), present in all tissues, are one of the best-studied classes of matrix molecules. Over 50 members of PG have been characterized and studied for their biological significance. PG consist of a protein core to which glycosaminoglycan chains are covalently linked. Glycosaminoglycans (GAGs) are natural heteropolysaccharides that are present in every mammalian tissue. They are composed of repeating disaccharide units that consist of either sulfated or non-sulfated monosaccharides. Their molecular size and the sulfation type vary based on tissue type. The major types of GAGs found in mammalian tissues are: hyaluronan, chondroitin sulfate, and dermatan sulfate, heparin and heparan sulfate, and keratan sulfate. GAG chains are covalently bound to serine residues of the PG protein core via a tetrasaccharide linkage, consisting of xylose, two galactose residues and glucuronic acid. This antibody, clone PG-4, recognizes antigen that is associated with extracellular matrices and surfaces of cultured human dermal fibroblasts and the surfaces of cultured human adult keratinocytes. This antibody recognize native epitopes located within glycosaminoglycan chains and is detects both chondroitin sulfate (Versican) and dermatan sulfate (Decorin and Biglycan) proteoglycans. Treatment of PG with chondroitinases is reported to abolish its immunoreactivity for all antigens. (Ref.: Afratis, N et al. (2012). FEBS Journal 279 (7); 1177-1197; Sorrell, JM et al. (1999). Histochem. J. 31 (8); 549-558).

특이성

Clone PG4 detects glycosaminoglycan in skin cells.

면역원

Crude membrane fractions from adult human dermal fibroblasts from breast skin.

애플리케이션

Anti-Glycosaminoglycan, skin specific, clone PG-4, Cat. No. MABT819, is a mouse monoclonal antibody that detects glycosaminoglycan and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Research Category
Cell Structure

Western Blotting Analysis: A representative lot detected Glycosaminoglycan in Western Blotting applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).

ELISA Analysis: A representative lot detected Glycosaminoglycan in ELISA applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).

Immunohistochemistry Analysis: A representative lot detected Glycosaminoglycan in Immunohistochemistry applications (Sorrell, J.M., et. al. (1999). Histochem J. 31(8):549-58).

품질

Evaluated by Immunohistochemistry (Paraffin) in human skin tissue.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected Glycosaminoglycan in human skin tissue.

표적 설명

~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash