MABT1475

Anti-Cytokeratin-8 Antibody, clone 1E8

• 동의어(들): CYK8, KRT8, Keratin, type II cytoskeletal 8, Keratin-8 (K8), Type-II keratin Kb8
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 1E8, monoclonal
• 분자량: calculated mol wt 53.7 kDa; observed mol wt ~54 kDa
• 정제법: using protein G
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): canine, monkey
• 포장: antibody small pack of 100
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; inhibition assay: suitable; western blot: suitable
• 동형: IgG2aκ
• 에피토프 서열: C-terminal
• 단백질 ID 수납 번호: NP_001243222
• UniProt 수납 번호: P05787
• 저장 온도: -10 to -25°C
• 유전자 정보: human ... KRT8(3856)

설명

특이성

Clone 1E8 is a mouse monoclonal antibody that detects human Cytokeratin-8. It targets an epitope within 12 amino acids from the C-terminal part.

면역원

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal of human Cytokeratin-8.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in HaCaT cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cytokeratin-8 in HaCaT cell lysate.

Tested Applications

Immunohistochemistry Applications: A representative lot detected Cytokeratin-8 in Immunohistochemistry application (Sfakianos, J.P., et al. (2020). Nat Commun. 11(1):2540).

Western Blotting Analysis: A representative lot detected Cytokeratin-8 in Western Blotting application (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

Immunocytochemistry Analysis: A representative lot detected Cytokeratin-8 in Immunocytochemistry application (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

Inhibition Assay: An Anti-Cytokeratin-8, clone 1E8 Fab decreased plasminogen binding to breast cancer cell lines in Inhibition Assay (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Keratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. They share a common structural organization: a central -helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. It is a type II, neutral to basic protein that together with cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73. Serine 23 is highly conserved in all type II keratins. Phosphorylation at serine 73 is reported to increase during cellular stress. However, under normal conditions serine 73 remains largely dephosphorylated. It can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.

물리적 형태

Purified mouse monoclonal antibody IgG2a in PBS without azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable