MABS2297

Anti-TPO Antibody, clone 47

• 동의어(들): MoAb47, Thyroid peroxidase
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 47, monoclonal
• 분자량: calculated mol wt 102.96 kDa; observed mol wt ~120 kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100
• 기술: ELISA: suitable; radioimmunoassay: suitable; western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_000538
• UniProt 수납 번호: P07202
• 저장 온도: 2-8°C
• 유전자 정보: human ... TPO(7173)

설명

특이성

Clone 47 is a mouse monoclonal antibody that specifically detects Thyroid peroxidase.

면역원

Human thyroid peroxidase (hTPO) purified from thyroid microsomes by immunoaffinity chromatography.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in human thyroid tissue extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Thyroid peroxidase (TPO) in human thyroid tissue extract.

Tested Applications

ELISA Analysis: A representative lot detected Thyroid peroxidase (TPO) in ELISA applications (Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8).

Radioimmunoassay: A representative lot detected Thyroid peroxidase (TPO) in Radioimmunoassay applications (Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

표적 설명

Thyroid peroxidase (Uniprot: P07202; also known as TPO) is encoded by gene TPO (Gene ID: 7173) in human. It is a single-pass type I membrane protein and has an Extracellular domain (19-846 aa), a Transmembrane domain ( 847-871 aa) and a Cytoplasmic domain (872-933 aa). It is primarily found in the thyroid gland and functions in the iodination of tyrosine residues in thyroglobulin and the formation of phenoxy-ester between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine (T3 and T4). TPO is an integral part of the complex process by which the thyroid gland regulates metabolism and other vital bodily functions. Defects in this gene can cause congenital hypothyroidism, congenital goitre and thyroid hormone organification defect IIA. Moreover, TPO is a common target of autoimmune attacks in various thyroid disorders, such as Hashimoto′s thyroiditis and Graves′ disease, which can lead to disruptions in hormone synthesis and subsequent health issues.
(Ref.: Ruf, J., et al. (1989). Endocrinology. 125(3):1211-8. (PMID: 2474433).

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable