MABS2267

Anti-ANT1/SLC25A4 Antibody, clone IF3F11

• 동의어(들): ADP/ATP carrier protein 1, ADP/ATP carrier protein, heart/skeletal muscle isoform T1, ADP/ATP translocase 1, Adenine nucleotide translocator 1, Solute carrier family 25 member 4
• UNSPSC 코드: 12352203
• NACRES: NA.46

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: IF3F11, monoclonal
• 분자량: calculated mol wt 33.06 kDa; observed mol wt ~33 kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100
• 기술: western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: Internal
• 단백질 ID 수납 번호: NP_001142
• UniProt 수납 번호: P12235
• 저장 온도: 2-8°C
• 유전자 정보: human ... SLC25A4(291)

설명

특이성

Clone IF3F11 is a mouse monoclonal antibody that detects Adenine nucleotide translocator 1 (ANT1/SLC25A4). It targets an epitope within 15 amino acids from the internal region.

면역원

His-tagged, full-length, recombinant human ADP/ATP translocase 1 (ANT1).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in Human heart tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ANT1/SLC25A4 in Human heart tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected ANT1/SLC25A4 in Human skeletal muscle and Human brain tissue extracts.

Western Blotting Analysis: A representative lot detected ANT1/SLC25A4 in Western Blotting applications (Lu, Y-W., et al. (2017). Mol Biol Cell. 28(11):1489-1500; Acoba, M.G., et al. (2021). Cell Rep. 34(11):108869).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

표적 설명

ADP/ATP translocase 1 (UniProt: P12235; also known as ADP/ATP carrier protein 1, ADP/ATP carrier protein, heart/skeletal muscle isoform T1 1 , Adenine nucleotide translocator 1, ANT 1, Solute carrier family 25 member 4) is encoded by the SLC25A4 (also known as AAC1, ANT1) gene (Gene ID: 291) in human. ANT1 is a multi-pass inner mitochondrial membrane protein with six transmembrane domains. It serves as an ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to provide energy for cellular functions. It is reported to cycle between the cytoplasmic-open state (c-state) and the matrix-open state (m-state) and operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic or matrix side of the inner mitochondrial membrane. It is also involved in mitochondrial uncoupling and mitochondrial permeability transition pore activity. ANT also acts as a regulator of mitophagy independently of ADP:ATP antiporter activity. It promotes mitophagy via interaction with TIMM44 that leads to inhibition of presequence translocase TIMM23, thereby promoting stabilization of PINK1. ANT1 deficiency has been implicated in various pathological states, including hypertrophic cardiomyopathy, mitochondrial myopathy, lactic acidosis, and progressive external opthalmoplegia. (Ref.: Hoshino, A., et al. (2019). Nature. 575(7782); 375-379; Lu, Y-W., et al. (2017). Mol. Biol. Cell. 28(11):1489-1506; Kawamaoto, H., et al. (2011). Hum. Mol. Genet. 20(15); 2964-2974).

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable