MABS1989

Anti-Osteocalcin Antibody, clone 4C5

clone 4C5, from mouse

• 동의어(들): Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: serum
• 항체 생산 유형: primary antibodies
• 클론: 4C5, monoclonal
• 종 반응성: human
• 포장: antibody small pack of 25 μL
• 기술: dot blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_954642.1
• UniProt 수납 번호: P02818
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... BGLAP(632)

설명

일반 설명

Osteocalcin (UniProt: P02818; also known as Bone Gla protein, BGP, Gamma-carboxyglutamic acid-containing protein) is encoded by the BGLAP gene (Gene ID: 632) in human. Osteocalcin is a member of the osteocalcin/matrix Gla protein family and can constitute 1 to 2% of the total bone protein. It is synthesized by osteoblasts and is a major component of the noncollagenous bone matrix. Osteocalcin is synthesized with a signal peptide (aa 1-23), a propetide (aa 24-51) that are cleaved to generate active secreted form of osteocalcin (aa 52-100). Osteocalcin displays a high affinity for calcium and binds strongly to apatite. Sixty to ninety percent of de novo synthesized osteocalcin is incorporated into the bone matrix where it binds to hydroxyapatite during matrix mineralization. The remainder of the osteocalcin is released into the circulation where it can be measured as a sensitive marker of bone formation. Osteocalcin found in serum is almost exclusively derived from the bone formation with little or no contribution from the resorption process. A major feature of osteocalcin is the presence of 3 vitamin K-dependent gamma-carboxyglutamic acid (Gla) residues, which are found in the first helical region and interact with the inter-calcium spacing in the HAP lattice. In humans, the percentage of the circulating osteocalcin that is not gamma-carboxylated is used as a biomarker of vitamin K status. Osteocalcin expression in osteoblasts is stimulated by insulin and adiponectin and osteocalcin enhances insulin sensitivity and differentiation in muscle. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Paget′s disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency. (Ref.: Kanazawa I (2015). World J Diabetes. 6(18): 1345 1354; Gundberg, CM et al. (2012). Adv Nutr 3 (2): 149-157).

특이성

Clone 4C5 detects all forms of human osteocalcin.

애플리케이션

Anti-Osteocalcin, clone 4C5, Cat. No. MABS1989, is a moue monocloanl antibody that detects osteocalcin and has been tested for use in Dot Blot.

Dot Blot Analysis: A representative lot detected 3xGlu, Glu17, and 3XGla peptides for human OCN (Courtesy of Mathieu Ferron, Ph.D., Montreal Clinical Research Institute, Montreal, Canada).

품질

Dot Blot Analysis: A 1:250 dilution from a representative lot detected Osteocalcin at a wide range of concentrations (0.8 to 1600 ng).

표적 설명

10.96 kDa calculated.

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.