MABS196

Anti-mTOR/FRAP Antibody, clone 22C2

clone 22C2, from mouse

• 동의어(들): Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapam
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 22C2, monoclonal
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_004949
• UniProt 수납 번호: P42345
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MTOR(2475)

설명

일반 설명

The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.

면역원

Linear peptide corresponding to human mTOR/FRAP.

애플리케이션

Detect the mTOR/FRAP protein using this Anti-mTOR Antibody, clone 22C2, validated for use in WB & IP.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Western Blot Analysis: A representative lot was used by an independent laboratory in neuroblastoma cells. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in Rho30 cell extracts. (Hosoi, H., et al. (1998). Molecular Pharmacology. 54:815–824.)

품질

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR/FRAP on 10 µg of Jurkat cell lysate.

표적 설명

~290 kDa observed

결합

Replaces: 04-385

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Jurkat cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable