MABS1268

Anti-MCRS1 Antibody, clone DON193B/C9

clone DON193B/C9, from rat

• 동의어(들): Microspherule protein 1, 58 kDa microspherule protein, Cell cycle-regulated factor p78, INO80 complex subunit J, MCRS2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: DON193B/C9, monoclonal
• 종 반응성: human
• 기술: western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_006328
• UniProt 수납 번호: Q96EZ8
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MCRS1(10445)

설명

일반 설명

Microspherule protein 1 (UniProt: Q96EZ8; also known as 58 kDa microspherule protein, Cell cycle-regulated factor p78, INO80 complex subunit J, MCRS1) is encoded by the MCRS1 (also known as INO80Q, MSP58) gene (Gene ID: 10445) in human. MCRS1 is a nucleolar protein that is detected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes. Its highest levels are reported in the early S phase and it becomes undetectable in the G2 phase. Four isoforms of MCRS1 have been reported that are produced by alternative splicing. MCRS1 contains a nucleolar localization motif (aa 113-123), a coiled-coil domain (aa 301-335), and a forkhead associated (FHA) domain (aa 363-419). MCRS1 exhibits transforming activity, which is suppressed by phosphatase and tensin homologue (PTEN). It can modulate the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 forms a complex with Mi-2 beta, and RET finger protein (RFP) and co-localize in the Nucleolus. This complex activates rRNA promoter activity. (Ref.: Shimono, K., et al. (2005). J. Biol. Chem. 280(47); 39436-39447).

특이성

Clone DON193B/C9 detects Microspherule protein 1 in human cells.

면역원

A mixture of GST-tagged full length recombinant human Microspherule protein 1 and GST-tagged C- and N-terminal recombinant fragments of human Microspherule protein 1.

Epitope: unknown

애플리케이션

Anti-MCRS1, clone DON193B/C9, Cat. No. MABS1268, is a rat monoclonal antibody that detects Microspherule protein 1 and has been tested for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected MCRS1 in human tonsil, human spleen, HeLa cell, and Ramos cell lysates (Courtesy of Giovanna Roncador, Ph.D., CNIO, Madrid, Spain).

품질

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected MCRS1 in 10 µg of HCT116 cell lysate.

표적 설명

~52 kDa observed; 51.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash