MABN2614

Anti-LOTUS Antibody, clone H24G11

• UNSPSC 코드: 12352203
• NACRES: NA.74

속성

• 생물학적 소스: hamster
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: H24G11, monoclonal
• 분자량: calculated mol wt 70.33 kDa; observed mol wt ~85 kDa
• 정제법: using protein G
• 종 반응성: mouse
• 포장: antibody small pack of 100 μL
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 동형: IgGκ
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_660105
• UniProt 수납 번호: Q8R555
• 배송 상태: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Crtac1(72832)

설명

일반 설명

Cartilage acidic protein 1 (UniProt: Q8R555; also known as 68 kDa chondrocyte-expressed protein, CEP-68, ASPIC, Protein CRTAC1-B, Lateral olfactory tract usher substance, LOTUS) is encoded by the Crtac1 (also known as Aspic1, Cep68) gene (Gene ID: 72832) in murine species. LOTUS is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form (aa 29-646). It is a membrane-bound and/or secreted protein that serves as an endogenous Nogo receptor-1 (NgR1) antagonist. It is shown to contribute to the formation of the lateral olfactory tract (LOT) axonal bundle. Endogenous Nogo-A signaling through NgR1 is shown to induce axonal branching. Developmental up-regulation of Nogo-A promotes collateral formation of the LOT during the late development period. It has been reported that axon growth inhibitors, which include Nogo proteins, myelin-associated glycoprotein (MAG), oligodendrocyte myelin glycoprotein (OMgp), and B lymphocyte stimulator (BLyS), bind to NgR1 and restrict functional recovery after damage to the adult central nervous system. LOTUS blocks the binding of these ligands to NgR1 and suppresses growth cone collapse. LOTUS knockout mice show significantly increased collateral branches of axons in the LOT. On the other hand, this branching is diminished in NgR1 knockout mice. A simultaneous loss of LOTUS and NgR1 is reported to rescue the abnormal increase in axonal branches. (Ref.: Iketani, M., et al. (2016). Sci. Rep. 6; 39586; Kurihara, Y., et al. (2014). Mol. Cell. Neurosci. 61; 211-218).

특이성

Clone H24G11 is a hamster monoclonal antibody that detects murine cartilage acidic protein 1B (LOTUS).

면역원

Homogenate of protein extract from developing lateral olfactory tract and the surrounding tissues of mouse.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in Mouse E14 brain tissue lysates.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LOTUS in Mouse E14 brain tissue lysates.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected LOTUS in Immunocytochemistry applications (Kurihara, Y., et al. (2014). Mol Cell Neurosci. 61:211-8; Sato, Y., et al. (2011). Science. 333(6043):769-73).

Immunohistochemistry Applications: A 1:50 dilutions from a representative lot detected LOTUS in E14 embryonic mouse tissue sections.

Western Blotting Analysis: A representative lot detected LOTUS in Western Blotting applications (Iketani, M., et al. (2016). Sci Rep. 6:39586).

Immunohistochemistry Applications: A representative lot detected LOTUS in Immunohistochemistry applications ( Sato, Y., et al. (2011). Science. 333(6043):769-73; Iketani, M., et al. (2016). Sci Rep. 6:39586).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-LOTUS, clone H24G11, Cat. No. MABN2614, is a hamster monoclonal antibody that detects Cartilage acidic protein 1 (LOTUS) and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

물리적 형태

Purified hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable