MABN2435

Anti-Peripherin-2 Antibody, clone 5H2

clone 5H2, from mouse

• 동의어(들): Retinal degeneration slow protein
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 5H2, monoclonal
• 종 반응성: bovine, mouse
• 포장: antibody small pack of 25 μg
• 기술: immunofluorescence: suitable; radioimmunoassay: suitable; western blot: suitable
• 동형: IgG1κ
• UniProt 수납 번호: P17810
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Prph2(19133)

설명

일반 설명

Peripherin-2 (UniProt: P17810; also known as Retinal degeneration slow protein) is encoded by the PRPH2 (also known as RDS) gene in bovine species. Peripherin-2 is a disulfide-linked homodimeric, multi-pass membrane protein that may function as an adhesion molecule involved in stabilization and compaction of outer segment disks in retina and is considered to be essential for disk morphogenesis. It is exclusively expressed in outer segments. Peripherin-2 can form homo- and heteromeric protein complexes in outer segment. The core homomeric peripherin-2 unit is the non-covalent tetramer, which can also assemble into covalently linked octamers and higher-order oligomers. The homomeric peripherin-2 complexes are essential for proper outer segment morphology and architecture. Peripherin-2 is also shown to form heteromeric complexes with its homolog ROM-1. Mutations in PRPH2 gene can lead to autosomal dominant retinitis pigmentosa (adRP) And a majority of these mutations are located within the large loop domain connecting the transmembrane domain 3 and 4 (Ref.: Bohm, S et al. (2017). Scientific Reports 7, Article number: 2321).

특이성

Clone 5H2 detects Peripherin-2 in bovine and mouse retina.

면역원

Epitope: C-terminus

Purified bovine whole peripherin.

애플리케이션

Anti-Peripherin-2, clone 5H2, Cat. No. MABN2435, is a highly specific mouse monoclonal antibody that targets Peripherin-2 and has been tested in Immunofluorescence, Radioimmunoassay, and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected Peripherin-2 in Western Blotting applications (Connell, G., et. al. (1991). Proc Natl Acad Sci USA. 88(3):723-6; Zhang, Y., et. al. (2009). J Cell Sci. 122(Pt 8):1192-200).

Radioimmunoassay Analysis: A representative lot detected Peripherin-2 in Radioimmunoassay applications (Connell, G., et. al. (1991). Proc Natl Acad Sci USA. 88(3):723-6).

Immunofluorescence Analysis: A representative lot detected Peripherin-2 in Immunoflourescence applications (Zhang, Y., et. al. (2009). J Cell Sci. 122(Pt 8):1192-200).

품질

Evaluated by Western Blotting in bovine retinal tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Peripherin-2 in 10 µg of bovine retinal tissue lysate.

표적 설명

~39 kDa observed; 38.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash