MABN2266

Anti-ARID1B Antibody, clone 3D9.1

clone 3D9.1, from mouse

• 동의어(들): AT-rich interactive domain-containing protein 1B, ARID domain-containing protein 1B, BRG1-associated factor 250b, BAF250B, BRG1-binding protein hELD/OSA1, Osa homolog 2, hOsa2, p250R
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 3D9.1, monoclonal
• 종 반응성: human, mouse
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2bκ
• NCBI 수납 번호: NP_059989
• UniProt 수납 번호: Q8NFD5
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ARID1B(57492)

설명

일반 설명

AT-rich interactive domain-containing protein 1B (UniProt: Q8NFD5; also known as ARID domain-containing protein 1B, BRG1-associated factor 250b, BAF250B, BRG1-binding protein hELD/OSA1, Osa homolog 2, hOsa2, p250R) is encoded by the ARID1B (also known as BAF250B, DAN15, KIAA1235, OSA2) gene (Gene ID: 57492) in human. ARID domain-containing protein 1B (ARID1B) is widely expressed with high levels in heart, skeletal muscle, and kidney. Four isoform of ARID1B have been reported that are produced by alternative splicing. Its ARID domain is localized to amino acids 1053-1144. ARID1B is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. It is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). ARID1B and ARID1A display about 60% homology, but have been reported to have opposing functions in cell cycle arrest and are mutually exclusive since individual SWI/SNF chromatin remodeling complexes can contain either ARID1A or ARID1B, but not both. ARID1B belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. Mutations in ARID1B gene are linked to the development of Coffin-Siris syndrome 1, a congenital multiple malformation syndrome with intellectual disability, coarse facial features, and malformations of the cardiac, gastrointestinal, genitourinary, and/or central nervous systems.

특이성

Clone 3D9.1 detects AT-rich interactive domain-containing protein 1B (ARID1B) in human and murine cells. It targets an epitope within 138 amino acids from the N-terminal half.

면역원

GST/His-tagged recombinant fragment corresponding to 138 amino acids from the N-terminal half of human AT-rich interactive domain-containing protein 1B.

애플리케이션

Anti-ARID1B, clone 3D9.1, Cat. No. MABN2266, is a highly specific mouse monoclonal antibody that targets AT-rich interactive domain-containing protein 1B and has been tested for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected ARID1B in HeLa, A431, HUVEC and NIH/3T3 cells.

품질

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARID1B in 10 µg of HeLa cell lysate.

표적 설명

~300 kDa observed; 236.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable