MABF951

Anti-IL-2R beta (CD122) Antibody, clone TM-beta1

clone TM-beta 1, from rat

• 동의어(들): Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, High affinity IL-2 receptor subunit beta, p70-75, CD122
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: TM-beta 1, monoclonal
• 종 반응성: mouse
• 포장: antibody small pack of 25 μL
• 기술: flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; neutralization: suitable; western blot: suitable
• 동형: IgG2bκ
• NCBI 수납 번호: NP_032394.1
• UniProt 수납 번호: P16297
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Il2Rb(16185)

설명

일반 설명

Interleukin-2 receptor subunit beta (UniProt: P16297; also known as IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, p70-75, CD122) is encoded by the Il2rb gene (Gene ID: 16185) in murine species. IL-2R is a non-covalent dimer of an alpha and a beta subunit. It can exist as a high affinity dimer, an intermediate affinity monomer of beta subunit, and as a low affinity monomer of alpha subunit. IL-2R beta is a single-pass type I membrane protein. The beta subunit is involved in receptor mediated endocytosis and it transduces the mitogenic signals of IL-2. IL-2R beta is synthesized with a signal peptide (aa 1-26), which is cleaved off to produce the mature form that has an extracellular domain (aa 27-240), a transmembrane domain (aa 241-268), and a long cytoplasmic domain (aa 269-539). IL-2R beta contains a WSXWS motif (aa 221-225), which is essential for its proper folding and thereby efficient intracellular transport. It also has a Box 1 motif (aa 281-289) that is required for JAK interaction and/or activation.

특이성

Clone TM-beta 1 is a rat monoclonal antibody that detects IL-2R beta subunit in murine species.

면역원

TART-mB whole cells

애플리케이션

Anti-IL-2R beta (CD122), clone TM-beta1, Cat. No. MABF951, is a rat monoclonal antibody that detects IL-2R beta and has been tested for use in Flow Cytometry, Function assay, Immunocytochemistry, Immunoprecipitation, Neutralizing, and Western Blotting.

Neutralizing Analysis: Freshly isolated mouse splenocytes pretreated with different amounts of MABF951, Anti-IL-2R beta (CD122), clone TM- 1 showed a co-relation of the amount of antibody used vs percent of cells stained with FITC-labeled antibody. In antibody titration, as the concentration of unlabeled anti-IL-2R beta (CD122) is decreased less inhibition is observed as a result.

Inhibits Activity/Function: A representative lot abolished the binding of IL2 ligand to IL-2R beta (CD122). (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8; Yokoyama, S., et. al. (2009). Proc Natl Acad Sci USA. 106(37):15849-54).

Flow Cytometry Analysis: A representative lot detected IL-2R beta (CD122) in Flow Cytometry applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Western Blotting Analysis: A representative lot detected IL-2R beta (CD122) in Western Blotting applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Immunoprecipitation Analysis: A representative lot immunoprecipitated IL-2R beta (CD122) in Immunoprecipitation applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Immunocytochemistry Analysis: A representative lot detected IL-2R beta (CD122) in Immunocytochemistry applications (Tanaka, T., et. al. (1991). J Immunol. 147(7):2222-8).

Research Category
Inflammation & Immunology

품질

Isotype testing: Identity Confirmation by Isotyping test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2b.

표적 설명

60.54 kDa calculated

물리적 형태

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2b in PBS without azide.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.