MABF3107

Anti-ULBP2 Antibody, clone BUMO1

• 동의어(들): ALCAN-alpha, N2DL-2, NKG2D ligand 2, NKG2DL2, Retinoic acid early transcript 1H, UL16-binding protein 2
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: BUMO1, monoclonal
• 분자량: calculated mol wt 27.37 kDa; observed mol wt ~N/A kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100
• 기술: ELISA: suitable; flow cytometry: suitable
• 동형: IgG1κ
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_079493
• UniProt 수납 번호: Q9BZM5
• 저장 온도: 2-8°C
• 유전자 정보: human ... ULBP2(80328)

설명

특이성

Clone BUMO1 is a mouse monoclonal antibody that detects UL16-binding protein 2 (ULBP2).

면역원

p815 murine mastocytoma cells expressing human ULBP2.

애플리케이션

Quality Control Testing

Evaluated by Flow Cytometry in C1R cells expressing ULBP2.

Flow Cytometry Analysis: 1.0 µg of this antibody detected ULBP2 in one million C1R cells expressing ULBP2.

Tested Applications

ELISA Analysis: A representative lot detected ULBP2 in ELISA applications (Waldhauer, I., et al. (2006). Cancer Res. 66(5):2520-6; Weil, S., et al. (2017). Front Immunol. 10:8:387).

Flow Cytometry Analysis: A representative lot detected ULBP2 in Flow Cytometry applications (Welte, S.A., et al. (2003). Eur J Immunol. 33(1):194-203; Waldhauer, I., et al. (2006). Cancer Res. 66(5):2520-6; Heinemann, A., et al. (2012). Cancer Res. 72(2):460-71).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

UL16-binding protein 2 (UniProt: Q9BZM5; also known as ALCAN-alpha, NKG2D ligand 2, N2DL-2, NKG2DL2, Retinoic acid early transcript 1H) is encoded by the ULBP2 (also known as N2DL2, RAET1H, UNQ463/PRO791) gene (Gene ID: 80328) in human. The UL16-binding proteins (ULBPs) belong to the family of MHC class I-related molecules that are targets of human CMV glycoprotein. ULBP2 is a GPI-anchor glycoprotein that is synthesized with a signal peptide (aa 1-25) and a propeptide (aa 217-246), which are subsequently cleaved off to produce the mature form. ULBP2 serves as a ligand of the activating immunoreceptor NKG2D and mediates natural kill (NK) cell cytotoxicity. It is detected in cell membrane and endoplasmic reticulum. A secreted for has also been described. Its expression is observed in cancer cells and in the fetus, but not in normal tissues. Tumor cells are reported to release the soluble form of ULBP2 by the action of metalloproteases, however, unlike membrane bound form, it does not down-regulate NKG2D on NK cells. Soluble form of ULBP2 is frequently detected in sera of patients with hematopoietic malignancies. NKG2D, frequently expressed on tumor cells, stimulate tumor immunity. Soluble forms of ULBPs are also reported to induce protein tyrosine phosphorylation, activation of Janus kinase 2, MAP kinase, and PI-3 kinase/Akt signaling. Clone BUMO-1 specifically detects ULBP2 and is suitable for multiple immunodetection applications. (Ref.: Waldhauer, I., and Steinle, A. (2006). Cancer Res 66(5); 2520-2526; Welte, SA., et al. (2003). Eur. J. Immunol. 33(1); 194-203; Sutherland, CL., et al. (2002). J. Immunol. 168(2); 671-679).

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable