MABF3074

Anti-SRF Antibody, clone 2C5

• 동의어(들): Serum response factor
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rat
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 2C5, monoclonal
• 분자량: calculated mol wt 51.25 kDa
• 정제법: using protein G
• 종 반응성: human, mouse
• 포장: antibody small pack of 100 μg
• 기술: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• 동형: IgG2aκ
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_065239
• UniProt 수납 번호: Q9JM73
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Srf(20807)

설명

일반 설명

Serum response factor (UniProt: Q9JM73; also known as SRF) is encoded by the Srf gene (Gene ID: 20807) in murine species. SRF is a transcription factor that binds to a serum response element (SRE) associated with a variety of genes, including immediate early genes such as c-Fos, FosB, JunB, Egr-1 and -2; neuronal genes such as nurr1 and nur77. It binds to DNA as a dimer and its DNA binding region is localized in amino acids 129-218. By regulating the expression of several genes, it controls cell growth and differentiation, neuronal transmission, and muscle development. Together with Myocardin Related Transcription Factor A (MRTFA) transcription coactivator, it controls the expression of genes regulating cytoskeleton development, morphogenesis, and cell migration. It is also required for cardiac differentiation and maturation. SRF is shown to be activated by a variety of agents, including serum, lysophosphatidic acid, lipopolysaccharide, 12-O-tetradecanoylphorbol-13-acetate (TPA), and cytokines. Keratinocytes in normal human and mouse skin are reported to express high levels of SRF, but its expression is strongly downregulated in the hyperproliferative epidermis of wounded and psoriatic skin. Loss of SRF is known to disrupt epidermal homeostasis. Mice lacking SRF in cardiac tissue display lethal cardiac defects between E10.5 and E13.5. (Ref.: Hu, X., et al. (2019). Nat. Commun. 10(1); 1695; Koegel, H., et al. (2009). J. Clin. Invest. 119(4); 899-910; Parlakian , A., et al (2004). Mol. Cell Biol. 24(12); 5281-5289; Chai, J., and Tarnawski, AS. (2002). J. Physiol. Pharmacol. 53(2); 147-157).

특이성

Clone 2C5 is a rat monoclonal antibody that detects murine Serum response factor (SRF).

면역원

Bacterially overexpressed His-tagged murine Serum response factor.

애플리케이션

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human psoriatic skin tissue sections.

Immunohistochemistry (Paraffin) Analysis (IHC(P)): A 1:50 dilution of this antibody detected Serum response factor (SRF) in human psoriatic skin tissue sections.

Tested Applications

Immunofluorescence Analysis: A representative lot detected SRF in Immunofluorescence applications (Koegel, H., et al. (2009). J Clin Invest. 119(4):899-910).

Immunohistochemistry Applications: A representative lot detected SRF in Immunohistochemistry applications (Koegel, H., et al. (2009). J Clin Invest. 119(4):899-910).

Western Blotting Analysis: A representative lot detected SRF in Western Blotting applications (Koegel, H., et al. (2009). J Clin Invest. 119(4):899-910).

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected SRF in Chromatin Immunoprecipitation applications (Hu, X., et al. (2019). Nat Commun. 10(1):1695).

Immunoprecipitation Analysis: A representative lot detected SRF in Immunoprecipitation applications (Hu, X., et al. (2019). Nat Commun. 10(1):1695).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SRF, clone 2C5, Cat. No. MABF3074, is a rat monoclonal antibody that detects serum response factor (SRF) and is used in Chromatin Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

물리적 형태

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable