MABF3048

Anti-MERS-CoV Nucleoprotein Antibody

• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 46, monoclonal
• 분자량: calculated mol wt 45.05 kDa; observed mol wt ~45 kDa
• 종 반응성: virus
• 포장: antibody small pack of 100 μL
• 기술: ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1κ
• UniProt 수납 번호: R9UM87
• 배송 상태: dry ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: Unknown ... N nucleocapsid protein(14254601)

설명

일반 설명

Nucleoprotein (UniProt: R9UM87; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 14254601) in Middle East respiratory syndrome-related coronavirus (MERS-CoV). The MERS-CoV is a positive-strand RNA virus of the betacoronavirus genus within the Coronaviridae family that causes severe respiratory syndrome in human. The mature MERS-CoV contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Nucleoprotein of MERS-CoV is a homooligomeric multifunctional protein that plays roles in genome encapsidation, RNA synthesis during replication, host translational shutoff, and innate immunity disruption. It packages the positive strand viral genome RNA into helical ribonucleocapsid and is involved in virion assembly through its interactions with the viral genome and membrane protein M. The nucleoprotein is reportedly organized into two folded domains known as the N-terminal domain (NTD) and the C-terminal domain (CTD) that are separated by a disordered region (LKR) containing a serine/ arginine stretch. Clone 46 displays specificity for MERS-CoV nucleoprotein and does not show cross-reactivity with nucleoprotein from other related viruses, including SARS coronavirus. (Ref.: Nguyen, THV., et al. (2019). Acta Crystallogr. D. Struct. Biol. 75(1); 8-15; Yamaoka, Y., et al. (2016). Front. Microbiol. 7; Article 509).

특이성

Clone 46 is a mouse monoclonal antibody that detects nucleoprotein from Middle East respiratory syndrome-related coronavirus (MERS-CoV).

면역원

His-tagged recombinant fragment corresponding to 292 amino acids from the nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting with recombinant MERS-CoV nucleoprotein.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected recombinant MERS-CoV Nucleoprotein.

Tested Applications

ELISA Analysis: A representative lot detected MERS-CoV Nucleoprotein in ELISA applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Western Blotting Analysis: A representative lot detected MERS-CoV Nucleoprotein in Western Blotting applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Immunoprecipitation Analysis: A representative lot immunoprecipitated MERS-CoV Nucleoprotein in Immunoprecipitation applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MERS-CoV Nucleoprotein, clone 46, Cat. No. MABF3048, is a mouse monoclonal antibody that detects MERS-CoV Nucleoprotein and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at +2°C to +8°C from date of receipt.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable