MABF2797

Anti-SARS-CoV-2 Spike RBD Antibody, clone 7D1-C12

• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies, primary antibodies
• 클론: 7D1-C12, monoclonal
• 분자량: calculated mol wt 141.18 kDa; observed mol wt ~200 kDa
• 정제법: using protein G
• 종 반응성: SARS coronavirus
• 포장: antibody small pack of 100 μg
• 기술: ELISA: suitable; immunoprecipitation (IP): suitable; neutralization: suitable; western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: Extracellular domain
• UniProt 수납 번호: P0DTC2
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: vaccinia virus ... NS1(956533)

설명

일반 설명

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. (Ref.: Abe, KT., et al. (2020). JCI Insight. 5(19); e142362).

특이성

Clone 7D1-C12 is a mouse monoclonal antibody that detects the spike glycoprotein of SARS-CoV-2. It targets an epitope within the receptor binding domain.

면역원

His-tagged recombinant fragment corresponding to 223 amino acids from receptor binding domain of SARS-CoV-2.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in lysates from HEK293T cells expressing full-length spike protein of SARS-CoV-2.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected spike glycoprotein in lysate from HEK293T expressing full-length SARS-CoV-2 spike protein, but not in lysate from wild type HEK393T cells.

Tested Applications

Western Blotting Analysis: A 1:400 dilution from a representative lot detected spike glycoprotein in whole cell lysate of HEK293T cells expressing full-length spike protein of SARS-CoV-2, but not in untransfected cells. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and E. Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

ELISA Analysis: Various dilutions of a hybridoma culture supernatant of clone 7D1-C12 detected native SARS-CoV-2 spike protein. ( (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and E. Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Neutralizing: A representative lot of this antibody reduced binding of SARS-CoV-2 RBD to ACE2 receptor in a Binding Interference Assay. ((Data courtesy: Kento Abe and Anne-Claude Gingras, University of Toronto, Canada; Abe, KT., et al. (2020). JCI Insight. 5(19); e142362).

Immunoprecipitation Analysis: A representative lot immunoprecipitated spike glycoprotein of SARS-CoV-2. (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and E. Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SARS-CoV-2 Spike RBD, clone 7D1-C12, Cat. No. MABF2797, is a mouse monoclonal antibody that detects Spike glycoprotein of SARS-CoV-2 and is tested for use in ELISA, Immunoprecipitation, Neutralizing, and Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG1 in PBS without azide.

저장 및 안정성

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable