MABF2790

Anti-CD96 Antibody, clone 6A6

• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 6A6, monoclonal
• 분자량: calculated mol wt 67.13 kDa
• 정제법: using protein G
• 종 반응성: mouse
• 포장: antibody small pack of 100 μg
• 기술: ELISA: suitable; blocking: suitable; flow cytometry: suitable; inhibition assay: suitable
• 동형: IgG2a
• 에피토프 서열: N-terminal
• 단백질 ID 수납 번호: NP_115854
• UniProt 수납 번호: Q3U0X8
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Cd96(84544)

설명

일반 설명

T-cell surface protein tactile (UniProt: Q3U0X8; also known as Cell surface antigen CD96, T cell-activated increased late expression protein, CD96) is encoded by the Cd96 gene (Gene ID: 85444) in murine species. CD96 is a single-pass type I, disulfide-linked, homodimeric, membrane glycoprotein protein of the poliovirus receptor (PVR, CD155)-nectin family that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 22-536), a transmembrane domain (aa 537-557), and a cytoplasmic domain (aa 558-602). It contains two Ig-like V-type 2 domains (aa 24-134 and 138-244) and one Ig-like C2-type domain (aa 250-355). These three Ig-like domains are separated from the transmembrane domain by a long region that is rich in proline, serine, and threonine. The cytoplasmic region contains a proline-rich tandem that is flanked by arginine and lysine residues and represents binding site for SH3 domain containing signaling components. CD96 is expressed mainly by cells of hematopoietic origin, in particular on T and NK cells. It is involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. It promotes NK cell-target adhesion by interacting with CD155 (PVR) present on target cells. Clone 6A6 is reported to bind to the first Ig domain of CD96 and competes with CD155 binding. It is shown to significantly reduce experimental lung metastases in murine models at low doses. (Ref.: Aguilera, AR., et al. (2018). OncoImmunology 7(5); e1424677; Georgiev, H., et al. (2018). Front. Immunol. 9; 1072; Meyer, D., et al. (2009). J. Biol. Chem. 284(4); 2235-2244).

특이성

Clone 6A6 is a rat monoclonal antibody that detects murine CD96. It targets an epitope within the extracellular domain

면역원

Purified recombinant Murine CD96.

애플리케이션

Quality Control Testing

Evaluated by Flow Cytometry in EL4 cells.

Flow Cytometry Analysis: 1 μg of this antibody detected CD96 in one million EL4 cells.

Tested Applications

Flow Cytometry Analysis: A representative lot detected CD96 in Flow Cytometry applications (Seth, S., et al. (2007). Biochem Biophys Res Commun. 364(4):959-65; Aguilera, A.R., et al. (2018). Oncoimmunology. 7(5):e1424677; Chiang, E.Y., et al. (2020). Eur J Immunol. 50(6):891-902).

ELISA Analysis: A representative lot detected CD96 in ELISA applications (Aguilera, A.R., et al. (2018). Oncoimmunology. 7(5):e1424677) .

Inhibition Assay: A representative lot of this antibody blocked CD96-CD155 interaction. (Seth, S., et al. (2007). Biochem Biophys Res Commun. 364(4):959-65; Aguilera, A.R., et al. (2018). Oncoimmunology. 7(5):e1424677; Georgiev, H., et al. (2018). Front Immunol. 9:1072).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CD96, clone 6A6, Cat. No. MABF2790, is a rat monoclonal antibody that detects CD96 (TACTILE) and is tested for use in ELISA, Flow Cytometry, Inhibition, Assay.

물리적 형태

Purified rat monoclonal antibody IgG2a in PBS without preservatives.

저장 및 안정성

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2