MABF2105

Anti-CD6 Antibody, clone UMCD6

clone UMCD6, from mouse

• 동의어(들): T-cell differentiation antigen CD6, T12, TP120
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: UMCD6, monoclonal
• 종 반응성: human
• 포장: antibody small pack of 25 μg
• 기술: dot blot: suitable; flow cytometry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_006716
• UniProt 수납 번호: P30203
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CD6(923)

설명

일반 설명

T-cell differentiation antigen CD6 (UniProt: P30203; also known as T12, TP120, CD6) is encoded by the CD6 gene (Gene ID: 923) in human. CD6 is a single-pass type I membrane protein that serves as a cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166. It contributes to signaling cascades triggered by activation of the TCR/CD3 complex and functions as a costimulatory molecule to promote T-cell activation and proliferation. It is also involved in the formation and maturation of the immunological synapse. CD6 has been detected on peripheral blood T-cells and on natural killer (NK) cells and is also detected in spleen, thymus, appendix, lymph nodes. CD6 is known to bind both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria and LPS binding leads to the activation of signaling cascades and down-stream MAP kinases. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 18-402), a transmembrane domain (aa 403-423), and a cytoplasmic domain (aa 424-668). Seven isoforms of CD6 have been reported that are produced by alternative splicing.

특이성

Clone UMCD6 specifically detects CD6 in human cells.

면역원

ST-1 T cells isolated from human knee synovium.

애플리케이션

Anti-CD6, clone UMCD6, Cat. No. MABF2105 is a mouse monoclonal antibody that detects CD6 and has been tested for use in Dot Blot, Flow Cytometry, Immunoprecipitation, Function Analysis, Inhibition assay.

Inhibition Analysis: A representative lot inhibited antigen-specific responses to tetanus toxoid, but not to the superantigen SEA. (Singer, N.G., et. al. (1996). Immunology. 88(4):537-43).

Induces Function: A representative lot showed strong synergistic effect with phorbol ester in inducing T cell activation and enhanced the autologous mixed lymphocyte reaction. (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).

Dot Blot Analysis: A representative lot detected CD6 in Dot Blot applications (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD6 in Immunoprecipitation applications (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).

Flow Cytometry Analysis: A representative lot detected CD6 in Immunoprecipitation applications (Singer, N.G., et. al. (1996). Immunology. 88(4):537-43; Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92; Bott, C.M., et. al. (1994). J Immunol. 153(1):1-9; Li, Y., et. al. (2017). Proc Natl Acad Sci USA. 114(10):2687-2692).

품질

Evaluated by Flow Cytometry in PMA stimulated Jurkat cells.

Flow Cytometry Analysis: A 1:1000 dilution of this antibody detected CD6 in one million Jurkat cells stimulated with PMA.

표적 설명

71.80 kDa calculated.

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.