MABF1968

Anti-Microglobulin beta2 Antibody, clone BBM.1

clone BBM.1, from mouse

• 동의어(들): B2M
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: BBM.1, monoclonal
• 종 반응성: human
• 포장: antibody small pack of 25 μg
• 기술: flow cytometry: suitable; immunohistochemistry: suitable (paraffin); inhibition assay: suitable; western blot: suitable
• 동형: IgG2bκ
• NCBI 수납 번호: NP_004039
• UniProt 수납 번호: P61769
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... B2M(567)

설명

일반 설명

Beta-2-microglobulin (UniProt: P61769) is encoded by the B2M (also known as CDABP0092, HDCMA22P) gene (Gene ID: 567) in human. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC) that is involved in the presentation of peptide antigens to the immune system. It is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved to generate the mature form. It also contains an Ig-like C1 type domain (aa 25-113). Beta-2-microglobulin lacks a transmembrane anchoring domain and is found in secreted as well as in complex. Serum beta-2-microglobulin levels are a reflection of cell turnover and its levels are shown to be elevated in fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin′s lymphoma. Its levels may be elevated either due to higher rates of synthesis as observed in HIV infection and malignancy or due to impaired renal filtration due to renal insufficiency.

특이성

Clone BBM.1 detects Beta-2-microglobulin in human.

면역원

MOLT-4, a Human acute lymphoblastic leukemia cell line.

애플리케이션

Anti-Microglobulin beta2, clone BBM.1, Cat. No. MABF1968, is a mouse monoclonal antibody that detects Microglobulin beta2 and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition assays, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected Microglobulin beta2 in Immunohistochemistry applications (Josson, S., et. al. (2011). Cancer Res. 71(7):2600-10).

Inhibition Analysis: A representative lot inhibited Microglobulin beta2 activity (Parham, P., et. al. (1983). J Biol Chem. 258(10):6179-86).

Flow Cytometry Analysis: A representative lot detected Microglobulin beta2 in Flow Cytometry applications (Drukker, M., et. al. (2012). Nat Biotechnol. 30(6):531-42).

Immunohistochemistry Analysis: A 1:50-1:1,000 dilution from a representative lot detected Microglobulin beta2 in human breast cancer and human tonsil tissues.

Research Category
Inflammation & Immunology

품질

Evaluated by Western Blotting in MOLT-4 cell lysates

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Microglobulin beta2 in 10 µg of MOLT-4 cell lysate.

표적 설명

~14 kDa observed; 13.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1