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Merck
모든 사진(4)

문서

MABE884

Sigma-Aldrich

Anti-Lin-28B, clone 17A2 Antibody

clone 17A2, from mouse

동의어(들):

Protein lin-28 homolog B, Lin-28B

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

17A2, monoclonal

종 반응성

human

기술

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG1κ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... LIN28B(389421)

일반 설명

Lin28 is a developmentally regulated RNA binding protein (RBP). It’s down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells (Viswanathan, 2008). Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers (Viswanathan, 2008). The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance (Wu, 2005). Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

특이성

This antibody reacts to both isoforms.

면역원

Recombinant protein corresponding to human Lin28B.

애플리케이션

Anti-Lin-28B, clone 17A2 | MABE884 is an antibody against Lin-28B for use in Western Blotting, Immunocytochemistry, Immunoprecipitation.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Lin28B in 10 µg of NTera2 cell lysate.
Immunocytochemistry Analysis: A representative lot detected Lin28B in K-562 cells (Prof. N. Kim, Seoul National University).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Lin28B in Huh7 cell lysate (Prof. N. Kim, Seoul National University).

품질

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Lin28B in 10 µg of HepG2 cell lysate.

표적 설명

~30 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at ~27 kDa and ~20 kDa

물리적 형태

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


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문서 라이브러리 방문

Julia K Polansky et al.
Scientific reports, 6, 19699-19699 (2016-01-30)
The common gamma (γc)-chain cytokine interleukin 15 (IL15) is a multifunctional immune-modulator which impacts the generation, maturation and activity of many cell types of the innate, as well as the adaptive immune system, including natural killer (NK) and CD8(+) T

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