MABE2033

Anti-GINS1 Antibody, clone 192B

• 동의어(들): DNA replication complex GINS protein PSF1;GINS complex subunit 1
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 192B, monoclonal
• 분자량: calculated mol wt 22.99 kDa; observed mol wt ~23 kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100 μg
• 기술: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1κ
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_066545
• UniProt 수납 번호: Q14691
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CD163(9332)

설명

일반 설명

DNA replication complex GINS protein PSF1 (UniProt: Q14691; also known as GINS complex subunit 1) is encoded by the GINS1 (also known as KIAA0186, PSF1) gene (Gene ID: 9837) in human. GINS1 is a component of the GINS (Go-Ichi-Ni-San) complex, a heterotetramer of GINS1, 2, 3, and 4, that plays an essential role in the initiation of DNA replication and progression of DNA replication forks. GINS complex is highly conserved in mammalian species. Its each subunit is composed of two types of structural domains: an -helix rich (A) domain and a β-strand rich (B) domain. GINS1 is highly expressed in undifferentiated cells and its deficiency in mice is shown to cause embryonic lethality by day 6.5. Its levels are significantly increased in several types of cancers, including lung, breast, and prostate cancers and several cancer cell lines. Mutations in GINS1 gene are known to cause immunodeficiency 5 (IMD55) that is characterized by chronic neutropenia, natural killer cell deficiency, and recurrent viral and bacterial infections, and most patients exhibit growth retardation. (Ref.: Hsieh, H-Y., et al. (2019). Cancer Sci. 111(7); 2400-2412; Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-2095).

특이성

Clone 192B is a mouse monoclonal antibody that detects Human DNA replication complex GINS protein PSF1 (GINS1).

면역원

Full-length recombinant human DNA replication complex GINS protein PSF1 (GINS1).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GINS1 in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GINS1 in MOLT-4 and Daudi cell lysates.

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated GINS1 in Immunoprecipitation applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Immunohistochemistry Applications: A representative lot detected GINS1 in Immunohistochemistry applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Western Blotting Analysis: A representative lot detected GINS1 in Western Blotting applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Immunofluorescence Analysis: A representative lot detected GINS1 in Immunofluorescence applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-GINS1, clone 192B, Cat. No. MABE2033, is a mouse monoclonal antibody that detects GINS complex subunit 1 and is tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1