MABE2022

Anti-GINS2/PSF2 Antibody, clone 78C

• 동의어(들): DNA replication complex GINS protein PSF2;GINS complex subunit 2
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 78C, monoclonal
• 분자량: calculated mol wt 21.43 kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100 μg
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2a
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: NP_057179
• UniProt 수납 번호: Q9Y248
• 배송 상태: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GINS2(51659)

설명

일반 설명

DNA replication complex GINS protein PSF2 (UniProt: Q9Y248; also known as GINS complex subunit 2) is encoded by the GINS2 (also known as PSF2, CGI-122, DC5, HSPC037) gene (Gene ID: 51659) in human. GINS/PSF2 is a component of the GINS complex, which is a heterotetramer of GINS 1, 2, 3, and 4 that are assembled with 1:1:1:1 stoichiometry. Each of these four subunits is essential for proper completion of DNA replication initiation and elongation. This complex plays an essential role in the initiation of DNA replication and progression of DNA replication forks. GINS complex binds preferentially to single-stranded DNA. At the G1/S transition, GINS is recruited to the origins of replication where it assembles with cell-division cycle protein Cdc45 and the minichromosome maintenance mutant (MCM)2-7 to form the Cdc45/Mcm2-7/GINS (CMG) complex, the presumed replicative helicase. GINS2 gene is reported to be frequently up-regulated in the cancer cells. Transfection of ETK-1 and HuH28 cells with PSF2 siRNA is shown to reduce the amount of transcript and suppress cell growth, indicating the important role of GINS2/PF2 in development of cholangiocarcinoma. (Ref.: Aparicio, T., et al. (2009). Nucl. Acids Res. 37(7); 2087-2095; Boskovic, J., et al. (2007). EMBO Rep. 8(7); 678-684; Obama, K., et al. (2005). Oncol. Rep. 14(3):701-706).

특이성

Clone 78C is a mouse monoclonal antibody that detects human DNA replication complex GINS protein PSF2 (GINS2/PSF2).

면역원

Recombinant human GINS2/PSF2 expressed in E. coli.

애플리케이션

Quality Control Testing

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected GINS2/PSF2 in A431 cells.

Tested Applications

Western Blotting Analysis: A representative lot detected GINS2/PSF2 in Western Blotting application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

Immunocytochemistry Analysis: A representative lot detected GINS2/PSF2 in Immunocytochemistry application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

Immunoprecipitation Analysis: A representative lot immunoprecipitated GINS2/PSF2 in Immunoprecipitation application (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-95).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-GINS2/PSF2, clone 78C, Cat. No. MABE2022, is a mouse monoclonal antibody that detects GINS/PSF2 and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable