MABE1805

Anti-Rabex-5 Antibody, clone G32

• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: G32, monoclonal
• 분자량: calculated mol wt 56.89 kDa; observed mol wt ~57 kDa
• 정제법: using protein G
• 종 반응성: human
• 포장: antibody small pack of 100 μg
• 기술: western blot: suitable
• 동형: IgG2aκ
• 에피토프 서열: N-terminal
• 단백질 ID 수납 번호: NP_001273989
• UniProt 수납 번호: Q9UJ41
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... RABGEF1(2742)

설명

일반 설명

Rab5 GDP/GTP exchange factor (UniProt: Q9UJ41; also known as RAP1, Rabaptin-5-associated exchange factor for Rab5, Rabex-5) is encoded by the RABGEF1 (also known as RABEX5) gene (Gene ID: 27342) in human. Rabex-5 is a guanine nucleotide exchange factor that catalyzes the exchange of GTP for GDP on the Rab5 subfamily of low molecular weight GTPases. It forms a tight complex with Rabaptin-5 that augments its exchange activity and generates GTP-bound, membrane-associated Rab5, which in turn binds Rabaptin-5 and stabilizes the Rabex-5- Rabaptin-5 complex on endosomes. It is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It can also act as a ubiquitin ligase. It binds to ubiquitin (Ub) through two independent ubiquitin-binding domains (UBDs). These UBDs determine a number of properties of Rabex-5, including its coupled monoubiquitination and interaction in vivo with ubiquitinated epidermal growth factor receptors. The first Ub binding region is localized in amino acids 2-49 and is named as RUZ domain (Rabex-5 ubiquitin binding zinc finger). The second Ub binding region is highly conserved and is localized in amino acids 48-74. In this domain Ala58 is shown to be critical for Ub binding and A58G mutagenesis causes significant reduction in its binding to Ub. (Ref.; Penengo, L., et al. (2006). Cell. 124. 1183-1195; Mattera, R., et al. (2006). J. Biol. Chem. 281(10); 6874-6883).

특이성

Clone G32 is a mouse monoclonal antibody that detects Rabex-5. It targets an epitope within 49 amino acids from the N-terminal region.

면역원

GST-tagged full length recombinant human Rabex-5.

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in lysates from HeLa cells.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Rabex-5 in lysates from HeLa cells transfected with either Rabex-5 siRNA or Lamin siRNA. Higher expression was detected in Lamin siRNA transfected HeLa cells.

Tested Applications

Western Blotting Analysis: A representative lot detected Rabex-5 in Western Blotting applications (Penengo, L., et al. (2006). Cell. 124(6): 1183-95).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Rabex-5, clone G32, Cat. No. MABE1805, is a mouse monoclonal antibody that detects Rebex-5 and is tested for use in Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable