MABE1008

Anti-TRRAP Antibody, clone 2TRR-1B3

ascites fluid, clone 2TRR-1B3, from mouse

• 동의어(들): Transformation/transcription domain-associated protein, 350/400 kDa PCAF-associated factor, PAF350/400, STAF40, TR-AP, Tra1 homolog
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: 2TRR-1B3, monoclonal
• 종 반응성: human
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_001231509
• UniProt 수납 번호: Q9Y4A5
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TRRAP(8295)

설명

일반 설명

Transformation/transcription domain-associated protein (UniProt Q9Y4A5; also known as 350/400 kDa PCAF-associated factor, PAF350/400, STAF40, TR-AP, Tra1 homolog) is encoded by the TRRAP (also known as PAF400) gene (Gene ID 8295) in human. Transformation/transcription domain-associated protein (TRRAP) is a cofactor component shared by several histone acetyltransferase (HAT) complexes, including SAGA (Spt-Ada-Gcn5-acetyltransferase), PCAF (P300/CBP-associated factor), and Tip60. TRRAP is an essential component of the histone epigenetic modifying mechinery that plays an important role in regulating cell-cycle progression. Trrap gene-knockdown or protein downregulation causes cell and mouse lethality, impaired spindle checkpoint and differentiation.

특이성

The immunogen sequence is present in both spliced isoforms of human TRRAP reported by UniProt (Q9Y4A5).

면역원

Epitope: N-terminal to nuclear localization signal in the TP53-interacting region.

Ovalbumin-conjugated linear peptide corresponding to a sequence from the TP53-interacting region of human TRRAP.

애플리케이션

Anti-TRRAP Antibody, clone 2TRR-1B3 is an antibody against TRRAP for use in Western Blotting, Immunoprecipitation.

Immunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated TRRAP from 1 mL (4.5 mg/mL) of HeLa nuclear extract (Courtesy of Dr. Mustapha OULAD-ABDELGHANI, IGBMC, France).
Immunoprecipitation Analysis: A representative lot immunodepleted TRRAP using nonhomologous end-joining/NHEJ-competent protein extracts prepared from human AAH-1 lymphoblastoid cells (Robert, F., et al. (2006). Mol. Cell. Biol. 26(2):402-412).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

품질

Evalulated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRRAP in 10 µg of HeLa nuclear extract.

표적 설명

~400 kDa observed. 437.6 kDa (isoform 1) and 434.4 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

물리적 형태

Mouse monoclonal IgG2aκ ascites without preservatives.

Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable