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Merck
모든 사진(2)

주요 문서

MABD117

Sigma-Aldrich

Anti-GATA Antibody3, clone 7B5

ascites fluid, clone 7B5, from mouse

동의어(들):

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
클론:
7B5, monoclonal
application:
IHC
WB
종 반응성:
human
기술:
immunohistochemistry: suitable
western blot: suitable
citations:

생물학적 소스

mouse

Quality Level

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

7B5, monoclonal

종 반응성

human

기술

immunohistochemistry: suitable
western blot: suitable

동형

IgG1

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... GATA3(2625)

일반 설명

GATA-binding factor 3 (GATA3), also known as Trans-acting T-cell-specific transcription factor GATA-3, is a transcription factor containing a zinc finger domain. GATA3 is primarily expressed in T cells, where it thought to participate in T-cell receptor gene activation through binding to enhancers. Analysis of conditional GATA3 knockout mice confirmed the critical role of GATA3 in helper T cell differentiation and proliferation. Cells expressing GATA3 develop the profile of Th2 cells, secreting IL-4, IL-5 and IL-13. Binding of GATA3 in the regulatory regions of the genes of these cytokines alters chromatin structure, increasing accessibility to other transcription factors. T cell receptor activation activates NFAT and other transcription factors that cooperate with GATA3 in inducing Th2 cell differentiation. Blocking GATA3 action has been suggested as a therapeutic strategy to treat asthma. GATA3 has also been implicated in developmental processes such as the development of the inner ear.

면역원

Purified recombinant fragment of human GATA3 expressed in E. Coli.

애플리케이션

Detect GATA using this mouse monoclonal antibody, Anti-GATA Antibody3, clone 7B5 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA3 in human small intestine tissue.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

품질

Evaluated by Western Blotting in GATA3-hIgGFc transfected HEK293 cell lysate.

Western Blot Analysis: A 1:500-2000 dilution of this antibody detected GATA3 in GATA3-hIgGFc transfected HEK293 cell lysate.

표적 설명

~75 kDa observed

물리적 형태

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
GATA3-hIgGFc transfected HEK293 cell lysate

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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