MABC1613

Anti-MUC1 Antibody, clone HMFG2

clone HMFG2, from mouse

• 동의어(들): Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epit
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: HMFG2, monoclonal
• 종 반응성: human
• 기술: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1λ
• NCBI 수납 번호: NP_002447.4
• UniProt 수납 번호: P15941
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MUC1(4582)

설명

일반 설명

Mucin-1 (UniProt: P15941; MUC-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227) is encoded by the MUC1 (also known as PUM) gene (gene ID: 4582) in human. The MUC-1 alpha subunit has cell adhesive properties and functions as protective layer on epithelial cells against bacteria and enzymes. It can act as both cell adhesion and an anti-adhesion protein. It forms a tight, non-covalent heterodimeric complex with the proteolytically-released beta-subunit. The beta subunit contains a C-terminal domain that is involved in cell signaling via phosphorylations and protein-protein interactions. It modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, it impacts directly or indirectly the Ras/MAPK pathway. MUC-1 also is involved in tumor progression and transcription through regulation of Tp53. MUC-1 is expressed in epithelial cells, breast, uterus, activated and unactivated T-cells. It is shown to be over-expressed in epithelial tumors, such as breast or ovarian cancer. MUC-1 is exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, it is internalized and recycled to the cell membrane. MUC-1 is highly glycosylated (N- and O-linked carbohydrates and sialic acid) and is O-glycosylated to a varying degree on serine and threonine residues within each tandem repeat, ranging from mono- to penta-glycosylation. The average density ranges from about 50% in human milk to over 90% in T47D breast cancer cells. Its interaction, via the tandem repeat region, with domain 1 of ICAM1 is implicated in cell migration and metastases. MUC1 serves as a serological clinical marker of breast cancer to monitor response to breast cancer treatment and disease recurrence. Lower levels over time may be indicative of a positive response to treatment.

특이성

Clone HMFG2 immunostains human breast cancer tissue. It can react with the unglycosylated peptide as well as several glycoforms of MUC1.

면역원

Delipidated milk fat globule extract followed 3 weeks later by epithelial cells cultured from milk (for 2 weeks).

애플리케이션

Detect Mucin-1 using this mouse monoclonal Anti-MUC1, clone HMFG2, Cat. No. MABC1613 that has been tested for use in Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Functional studies, and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MUC1 in human lung and human pancreas tissue.

Western Blotting Analysis: A representative lot detected MUC1 in Western Blotting applications (Kaur, S., et. al. (2014). PLoS One. 9(3):e92742; Schroeder, J.A., et. al. (2001). J Biol Chem. 276(16):13057-64).

Affects Function: A representative lot detected MUC1 in Affects Function applications (Schettini, J., et. al. (2012). Cancer Immunol Immunother. 61(11):2055-65).

Immunoprecipitation Analysis: A representative lot detected MUC1 in Immunoprecipitation applications in (Schroeder, J.A., et. al. (2001). J Biol Chem. 276(16):13057-64).

Immunohistochemistry Analysis: A representative lot detected MUC1 in Immunohistochemistry applications in (Sakurai, J., et. al. (2007). Eur J Histochem. 51(2):95-102).

Research Category
Apoptosis & Cancer

품질

Evaluated by Immunohistochemistry in human breast cancer tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected MUC1 in human breast cancer tissue.

표적 설명

122.10 kDa calculated.

물리적 형태

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1