추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
1G10.2, monoclonal
종 반응성
human, mouse, rat
포장
antibody small pack of 25 μg
제조업체/상표
Chemicon®
기술
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG2a
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... GAD1(2571)
관련 카테고리
일반 설명
Gutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65 kDa and 67 kDa, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, 1999). GAD65 is an amphiphilic, membrane-anchored protein (585 a.a.), encoded on human chromosome 10, and is responsible for vesicular GABA production. GAD67 is cytoplasmic (594 a.a.), encoded on chromosome 2, and seems to be responsible for significant cytoplasmic GABA production. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, 1995). GAD67 expression seems to be particularly plastic and can change in response to experimental manipulation (for example neuronal stimulation or transection) or disease progression and emergent disorders like schizophrenia (Volk, 2000). Colocalization of the two GAD isoforms also shows changes in GAD65/GAD67 distributions correlated with certain disease states such as IDDM and SMS.
특이성
Anti-GAD67 Antibody, clone 1G10.2 reacts with the 67kDa isoform of Glutamate Decarboxylase (GAD67) of rat, mouse and human origins, other species not yet tested. No detectable cross-reactivity with GAD65 by Western blot on rat brain lysate when compared to blot probed with AB1511 that reacts with both GAD65 & GAD67.
면역원
Recombinant GAD67 protein
애플리케이션
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neurotransmitters & Receptors
Neuronal & Glial Markers
This Anti-GAD67 Antibody, clone 1G10.2 is validated for use in IH, IH(P), WB for the detection of GAD67. This Anti GAD antibody has more than 75 product citations.
Western Blot: A 1:5,000-1:10,000 dilution was used on a previous lot using ECL on rat and mouse brain lysate.
Immunohistochemistry on 4% paraformaldehyde fixed mouse and rat tissue: 1:1,000-1:5,000. The antibody has also worked well on human tissue fixed for 10 minutes in 4% PFA at 4°C or 100% ethanol at +20°C or 100% acetone at -20°C at dilutions of 1:2,500-1:5,000.
A polyclonal to GAD67 is also available as AB5992.
Optimal working dilutions must be determined by end user.
Immunohistochemistry on 4% paraformaldehyde fixed mouse and rat tissue: 1:1,000-1:5,000. The antibody has also worked well on human tissue fixed for 10 minutes in 4% PFA at 4°C or 100% ethanol at +20°C or 100% acetone at -20°C at dilutions of 1:2,500-1:5,000.
A polyclonal to GAD67 is also available as AB5992.
Optimal working dilutions must be determined by end user.
품질
Routinely evaluated by immunohistochemistry by SKNSH cell lysate.
Immunohistochemistry(paraffin) Analysis:
Representative staining pattern and morphology of GAD67 in somatosensory 1, barrel field of the mouse cerebral cortex. All brown spots are Lamina VI a neurons. No Epitope retrieval was necessary. This lot of the antibody was diluted to 1:500, using IHC Select Detection with HRP-DAB.
Optimal Staining pattern/morphology of GAD67: Mouse Brain.
Immunohistochemistry(paraffin) Analysis:
Representative staining pattern and morphology of GAD67 in somatosensory 1, barrel field of the mouse cerebral cortex. All brown spots are Lamina VI a neurons. No Epitope retrieval was necessary. This lot of the antibody was diluted to 1:500, using IHC Select Detection with HRP-DAB.
Optimal Staining pattern/morphology of GAD67: Mouse Brain.
표적 설명
67 kDa
결합
Replaces: AB5992; AB5862P
물리적 형태
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a in buffer containing 0.1% sodium azide
저장 및 안정성
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
분석 메모
Control
SKNSH cell lysate (human neuroblastoma), mouse cerebral cortex.
SKNSH cell lysate (human neuroblastoma), mouse cerebral cortex.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Deletion of GAD67 in dopamine receptor-1 expressing cells causes specific motor deficits.
Heusner, CL; Beutler, LR; Houser, CR; Palmiter, RD
Genesis (2000)
Fu-Quan Huo et al.
Cerebral cortex (New York, N.Y. : 1991), 19(6), 1263-1272 (2008-11-05)
The ventrolateral orbital cortex (VLO) is part of an endogenous analgesic system, consisting of the spinal cord-thalamic nucleus submedius-VLO periaqueductal gray (PAG)-spinal cord loop. The present study examined morphological connections of GABAergic (gamma-aminobutyric acidergic) neurons and serotonergic projection terminals from
Hanno S Meyer et al.
Cerebral cortex (New York, N.Y. : 1991), 20(10), 2277-2286 (2010-06-11)
This is the second article in a series of three studies that investigate the anatomical determinants of thalamocortical (TC) input to excitatory neurons in a cortical column of rat primary somatosensory cortex (S1). Here, we report the number and distribution
B Fritsch et al.
Neuroscience, 163(1), 415-429 (2009-06-23)
An acute brain insult such as traumatic head/brain injury, stroke, or an episode of status epilepticus can trigger epileptogenesis, which, after a latent, seizure-free period, leads to epilepsy. The discovery of effective pharmacological interventions that can prevent the development of
Temporal patterns of fos expression in the dentate gyrus after spontaneous seizures in a mouse model of temporal lobe epilepsy.
Peng, Z; Houser, CR
The Journal of Neuroscience null
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