추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
A2b1, monoclonal
종 반응성
human, rat, mouse
제조업체/상표
Chemicon®
기술
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
적합성
not suitable for immunohistochemistry (Paraffin)
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... PTEN(5728) , TEP1(7011)
특이성
Specifically recognizes PTEN*, a tumor suppressor protein located on human chromosome 10 (Steck, ′97; Li, ′97; Rhei, ′97) that is often mutated in various types of advanced cancers (Rhei, ′97). The PTEN protein exhibits protein phosphatase activity (Myers, ′97) and can suppress the growth of glioma cells (Furnari, ′97). The antibody recognizes human and mouse PTEN and is specific for the C-terminus. Other species not tested. Reactivity with human fibroblasts, breast epithelial and lung cancer cell lines has been confirmed by western blot, and antibody specificity has been confirmed by peptide blocking studies.
*PTEN is covered under U.S. patents 6,262,242, 6,482,795 and U.S. patent application 10/299,003.
*PTEN is covered under U.S. patents 6,262,242, 6,482,795 and U.S. patent application 10/299,003.
면역원
Anti-PTEN was generated by immunization with a 10 amino acid peptide corresponding to amino acids 387-400 near the C-terminus of human PTEN.
Epitope: C-terminus
애플리케이션
Anti-PTEN Antibody, C-terminus, clone A2b1 is an antibody against PTEN for use in IC, IH, IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Western blot 1:100 (55 kDa)
Immunocytochemistry
Immunohistochemistry: 1:20 to 1:50 for acetone-fixed, fresh frozen tissue. Not effective for paraffin sections.
Immunoprecipitation 1:10 to 1:20
Protocol for immunohistochemistry of frozen tissue sections on glass slides.
1. Fix sections for 5 minutes with cold acetone.
2. Air dry
3. Wash sections with PBS
4. Add blocking solution (horse serum) for 20 minutes
5. Add diluted PTEN antibody and incubate for 1 hour at room temperature.
6. Wash with PBS
7. Add biotinylated secondary antibody (horse anti-mouse, or equivalent).
8. Incubate for 30 minutes at room temperature.
9. Wash with PBS
10. Detect with ABC (Avidin-Peroxidase-Complex) or equivalent.
11. Wash with PBS
12. Develop with DAB substrate.
13. Hematoxylin dye may be used as a counterstain for visualizing nuclei.Breast tumor tissue gives good staining. Prostate tissue does not stain.Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunohistochemistry: 1:20 to 1:50 for acetone-fixed, fresh frozen tissue. Not effective for paraffin sections.
Immunoprecipitation 1:10 to 1:20
Protocol for immunohistochemistry of frozen tissue sections on glass slides.
1. Fix sections for 5 minutes with cold acetone.
2. Air dry
3. Wash sections with PBS
4. Add blocking solution (horse serum) for 20 minutes
5. Add diluted PTEN antibody and incubate for 1 hour at room temperature.
6. Wash with PBS
7. Add biotinylated secondary antibody (horse anti-mouse, or equivalent).
8. Incubate for 30 minutes at room temperature.
9. Wash with PBS
10. Detect with ABC (Avidin-Peroxidase-Complex) or equivalent.
11. Wash with PBS
12. Develop with DAB substrate.
13. Hematoxylin dye may be used as a counterstain for visualizing nuclei.Breast tumor tissue gives good staining. Prostate tissue does not stain.Optimal working dilutions must be determined by end user.
표적 설명
55 kDa
결합
Replaces: 04-409
물리적 형태
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
저장 및 안정성
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Breast tumor tissue
Purified active kinase is Catalogue Number 14-488
Breast tumor tissue
Purified active kinase is Catalogue Number 14-488
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Carsten B Schmidt-Weber et al.
European journal of immunology, 32(4), 1196-1204 (2002-04-05)
The phosphatidylinositol phosphatase gene PTEN is a dual specific phosphatase acting on phospho amino acids but also on three phosphorylated inositol phospholipids. Present results demonstrate that PTEN is inducible by costimulatory signals in human CD4(+) T cells. PTEN expression was
Delira Robbins et al.
FEBS letters, 586(23), 4108-4113 (2012-11-06)
Our recent studies suggest a unique role of p53 during the early stage of cancer development. However, how p53 activation is regulated during TPA treatment remains elusive. We used murine skin epidermal JB6 promotion-sensitive (P+) and promotion resistant (P-) cells
Janice B B Lam et al.
PloS one, 4(3), e4968-e4968 (2009-03-26)
Adiponectin is an adipokine possessing beneficial effects on obesity-related medical complications. A negative association of adiponectin levels with breast cancer development has been demonstrated. However, the precise role of adiponectin deficiency in mammary carcinogenesis remains elusive. In the present study
Danyu Cui et al.
Molecular medicine reports, 16(5), 7813-7820 (2017-09-26)
A number of short noncoding microRNAs (miRs) have been demonstrated to be highly expressed in many kidney diseases such as renal cancer and lupus nephritis (LN); however, these results have not been extensively investigated. The aim of the present study
Joseph O Humtsoe et al.
Molecular and cellular biology, 30(7), 1593-1606 (2010-02-04)
Endothelial cell (EC) migration, cell-cell adhesion, and the formation of branching point structures are considered hallmarks of angiogenesis; however, the underlying mechanisms of these processes are not well understood. Lipid phosphate phosphatase 3 (LPP3) is a recently described p120-catenin-associated integrin
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