MAB3776

Anti-MDM2 Antibody, clone SMP14

clone SMP14, Chemicon^®, from mouse

• 동의어(들): Murine Double Minute 2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: SMP14, monoclonal
• 종 반응성: rat, human, mouse
• 제조업체/상표: Chemicon^®
• 기술: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable
• 동형: IgG1
• NCBI 수납 번호: NM_002392.2; NM_006878.2; NM_006879.2; NM_006881.2; NM_006882.2
• UniProt 수납 번호: Q00987
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MDM2(4193) ; mouse ... Mdm2(17246) ; rat ... Mdm2(314856)

설명

일반 설명

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

특이성

Recognizes human MDM2.

면역원

Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.

애플리케이션

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

RNA Binding Protein (RBP)

Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.

Western blot

Immunohistochemistry (Frozen and Paraffin-embedded sections)

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

물리적 형태

Format: Purified

Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide

분석 메모

Control
POSITIVE CONTROL: Breast carcinoma

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable