추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
1MOD-1A9, monoclonal
종 반응성
mouse, human
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... CBX1(10951)
일반 설명
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
특이성
Reacts with Heterochromatin protein-1 b (HP1b).
면역원
Recombinant mouse HP1beta.
애플리케이션
Use Anti-Heterochromatin Protein-1 β Antibody, clone 1MOD-1A9 (Mouse Monoclonal Antibody) validated in ELISA, ICC, IHC, IP, WB to detect Heterochromatin Protein-1 β also known as HP1b.
Western blot: 1:500-1:5,000
Immunofluorescence: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
Immunoprecipitation: 1:500 - 1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by the end user.
Immunofluorescence: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
Immunoprecipitation: 1:500 - 1:5,000
ELISA: 1:500-1:5,000
Optimal working dilutions must be determined by the end user.
표적 설명
28 kDa
분석 메모
Control
HeLa, 293 or NIH/3T3 cell lysates
HeLa, 293 or NIH/3T3 cell lysates
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Sarah Herberg et al.
Scientific reports, 5, 14236-14236 (2015-09-22)
Transposable elements in the genome are generally silenced in differentiated somatic cells. However, increasing evidence indicates that some of them are actively transcribed in early embryos and the proper regulation of retrotransposon expression is essential for normal development. Although their
Kyoko Hiragami-Hamada et al.
Nature communications, 7, 11310-11310 (2016-04-20)
Histone H3 trimethylation of lysine 9 (H3K9me3) and proteins of the heterochromatin protein 1 (HP1) family are hallmarks of heterochromatin, a state of compacted DNA essential for genome stability and long-term transcriptional silencing. The mechanisms by which H3K9me3 and HP1
Epigenetic regulation of myogenic gene expression by heterochromatin protein 1 alpha.
Sdek, P; Oyama, K; Angelis, E; Chan, SS; Schenke-Layland, K; MacLellan, WR
Testing null
Erik Müllers et al.
Aging cell, 16(3), 575-584 (2017-03-28)
In response to DNA damage, a cell can be forced to permanently exit the cell cycle and become senescent. Senescence provides an early barrier against tumor development by preventing proliferation of cells with damaged DNA. By studying single cells, we
Annika Baude et al.
Nucleic acids research, 44(5), 2214-2226 (2016-01-02)
We have recently identified lens epithelium-derived growth factor (LEDGF/p75, also known as PSIP1) as a component of the homologous recombination DNA repair machinery. Through its Pro-Trp-Trp-Pro (PWWP) domain, LEDGF/p75 binds to histone marks associated with active transcription and promotes DNA
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