추천 제품
생물학적 소스
mouse
Quality Level
100
300
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
JB1A (J10), monoclonal
종 반응성
human
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG1
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ITGB1(3688)
일반 설명
The primary function of the integrin family is to mediate cell-cell and cell-matrix adhesion interaction. CD29 is ubiquitous, with broad tissue distribution, but is not expressed on erythrocytes and is expressed only weakly on granulocytes. Integrins play an important role in cell adhesion and migration, and their normal function is critical in the induction and maintenance of cell differentiation. Loss or downregulation of CD29 has been proposed to be one of the general pathways through which carcinoma cells may acquire a more invasive and differentiated phenotype. Furthermore, loss or downregulation of integrin occurs relatively frequently in poorly differentiated colorectal and breast carcinomas as determined by immunohistochemistry.
특이성
Reacts with the human integrin beta 1. Note: integrin beta 1 is expressed in all tissues/cell types except erythrocytes. The epitope recognized by JB1a was recently mapped to residues 82-87 of the mature beta 1 integrin chain. In this study Mn++ was found to inhibit binding of JB1a both to mature beta 1 integrin and to a synthetic peptide corresponding to residues 82-87{Ni, 1998}.
면역원
Epitope: a.a. 82-87
Purified beta 1 integrin and Jurkat cells
애플리케이션
Inhibition of Jurkat binding to collagen and fibronectin:
1:100 Note: epitope is trypsin sensitive; cells should be prepared with PBS + EDTA (2mM) or other non-enzymatic methods. Blocking will not occur with trypsinized cells with JB1A.
Immunoprecipitation: 1:250-500
Western blotting (reducing, 115 kDa; non-reducing, 130 kDa): 1:1000-2000
Note: binding of antibody may be inhibited by magnesium ion concentrations exceeding 0.5mM.
Immunocytochemistry/Immunohistochemistry: JB1A can be used on unfixed cells in flow cytometry and culture {Akimov, 2001; Blood 98(5):1567-1576}. Often times fixing with 2-3% PFA for 5 minutes after primary antibody incubation will assist in obtain strong signals since it fixes the primary antibodies to the tissues.
FACS: JB1A has been primarily used against live cells at 1:200-1:500 followed by goat anti-mouse conjugated secondaries
Optimal working dilutions must be determined by end user.
1:100 Note: epitope is trypsin sensitive; cells should be prepared with PBS + EDTA (2mM) or other non-enzymatic methods. Blocking will not occur with trypsinized cells with JB1A.
Immunoprecipitation: 1:250-500
Western blotting (reducing, 115 kDa; non-reducing, 130 kDa): 1:1000-2000
Note: binding of antibody may be inhibited by magnesium ion concentrations exceeding 0.5mM.
Immunocytochemistry/Immunohistochemistry: JB1A can be used on unfixed cells in flow cytometry and culture {Akimov, 2001; Blood 98(5):1567-1576}. Often times fixing with 2-3% PFA for 5 minutes after primary antibody incubation will assist in obtain strong signals since it fixes the primary antibodies to the tissues.
FACS: JB1A has been primarily used against live cells at 1:200-1:500 followed by goat anti-mouse conjugated secondaries
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin β1 Antibody, a.a. 82-87, clone JB1A (a.k.a. J10) is validated for use in FC, IH, IP, FUNC, WB for the detection of Integrin β1.
표적 설명
88 kDa
물리적 형태
Ascites fluid containing no preservatives.
Unpurified
저장 및 안정성
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Human lung, liver, and skeletal muscle tissues
Human lung, liver, and skeletal muscle tissues
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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