추천 제품
제품명
Ready-to-Assay LPA3 Lysophospholipid Receptor Frozen Cells,
생물학적 소스
human
제조업체/상표
Ready-to-Assay
기술
calcium flux assay: suitable
NCBI 수납 번호
검출 방법
fluorometric
일반 설명
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Lysophosphatidic acid (LPA) is a lysophospholipid produced by activated platelets that inhibits adenylate cyclase and stimulates DNA synthesis, changes in cell morphology, and increases in intracellular calcium in a variety of cultured mammalian cells. A family of three GPCRs, LPA1, LPA2 and LPA3, mediates the biological effects of LPA (Contos et al., 2000). LPA3 is expressed primarily in testes, prostate, heart and pancreas. Mice lacking LPA3 have low expression of uterine COX-2 and display decreased uterine implantation and embryo spacing (Ye et al., 2005). Millipore′s cloned human LPA3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant LPA3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at LPA3.
Lysophosphatidic acid (LPA) is a lysophospholipid produced by activated platelets that inhibits adenylate cyclase and stimulates DNA synthesis, changes in cell morphology, and increases in intracellular calcium in a variety of cultured mammalian cells. A family of three GPCRs, LPA1, LPA2 and LPA3, mediates the biological effects of LPA (Contos et al., 2000). LPA3 is expressed primarily in testes, prostate, heart and pancreas. Mice lacking LPA3 have low expression of uterine COX-2 and display decreased uterine implantation and embryo spacing (Ye et al., 2005). Millipore′s cloned human LPA3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant LPA3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at LPA3.
세포주 설명
- GPCR Cell Lines
- Host cells: Chem-1
애플리케이션
Human LPA3 / EDG7 GPCR frozen cells for Calcium Flux FLIPR Assays.
생화학적/생리학적 작용
- GPCR Class: A
- Protein Target: LPA3/ EDG7
- Target Sub-Family: Lysophospholipid
성분
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
면책조항
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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