일반 설명
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
P2Y1 is a GPCR that binds to ADP and ATP to activate Gq. A wide variety of cells and tissues express P2Y1. Vascular endothelium and smooth muscle express P2Y1, and mediate vascular tone both in the resting state and in thrombosis, when platelet-derived ATP and ADP are present at high concentrations. In addition, activation of P2Y1 expressed on platelets leads to ADP-induced shape changes and aggregation (Ralevic and Burnstock, 1998). Millipore′s cloned human P2Y1-expressing cell line is made in the 1321N1 host, which supports high levels of recombinant P2Y1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at P2Y1.
P2Y1 is a GPCR that binds to ADP and ATP to activate Gq. A wide variety of cells and tissues express P2Y1. Vascular endothelium and smooth muscle express P2Y1, and mediate vascular tone both in the resting state and in thrombosis, when platelet-derived ATP and ADP are present at high concentrations. In addition, activation of P2Y1 expressed on platelets leads to ADP-induced shape changes and aggregation (Ralevic and Burnstock, 1998). Millipore′s cloned human P2Y1-expressing cell line is made in the 1321N1 host, which supports high levels of recombinant P2Y1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at P2Y1.
세포주 설명
- GPCR Cell Lines
- Host cells: 1321N1
애플리케이션
Human P2Y1 GPCR frozen cells for Calcium Flux FLIPR Assays.
생화학적/생리학적 작용
- GPCR Class: A
- Protein Target: P2Y1
- Target Sub-Family: P2Y/ Purinergic (metabotropic)
성분
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
면책조항
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.