CC145

Rat Laminin

from rat bladder carcinoma, liquid, suitable for cell culture

• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

속성

• product name: Rat Laminin-5,
• 생물학적 소스: rat
• Quality Level: 100
• 분석: >95% (SDS-PAGE)
• 형태: liquid
• 제조업체/상표: Chemicon^®
• 기술: cell culture | mammalian: suitable
• 입력: sample type neural stem cell(s); sample type induced pluripotent stem cell(s); sample type epithelial cells; sample type pancreatic stem cell(s); sample type: mouse embryonic stem cell(s); sample type mesenchymal stem cell(s); sample type hematopoietic stem cell(s)
• UniProt 수납 번호: P70636
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: rat ... Lama5(140433)

설명

일반 설명

Laminin-5 is a basement membrane extracellular matrix macromolecule that provides an attachment substrate for both adhesion and migration in a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. It is a natural substrate for cells in contact with basement membranes, including epidermal and oral keratinocytes, gastro-intestinal tract, mammary and prostate epithelial cells. These cells, compared to fibronectin, collagen, or vitronectin, will adhere to Laminin-5 faster and spread to a larger extent. Laminin-5 can be used, in most applications, at coating concentrations in the 1 mg/ml or lower range, approximately 10-fold lower than most extracellular matrix macromolecules. Laminin-5 is attracting attention as a substrate that may stimulate carcinoma cell migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas. Receptors for Laminin-5 include integrins a3b1 and a6b4. The latter integrin is specialized in forming hemidesmosomes, which provide resistance to mechanical stress in many epithelia, including skin and gut. More recently, cell surface proteoglycans, such as syndecan-4, have been identified as receptors for Laminin-5. The structural and functional properties of Laminin-5 are well conserved across species. For instance, human cells adhere equally well to human and rat laminin-5. For most purposes, human and rat Laminin-5 are interchangeable, likely based on the fact that integrin receptor specificity is largely conserved across species.

Product Source: Rat bladder carcinoma cell line 804G {Koshikawa, N et al. (2000) J. Cell Biol. 148:615-624.

애플리케이션

Recommended coating concentrations: 1 μg/mL for cell adhesion assays, 0.2 μg/mL for cell migration assays.

Optimal dilutions must be determined by end user

물리적 형태

Purified protein supplied in 20mM Tris, pH 7.5, 0.005% Brij-35, and 1% CHAPS containing no preservatives.

저장 및 안정성

Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

기타 정보

Concentration: Variable

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable