CC077

Human Collagen Type V

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

• 동의어(들): Collagen Type V
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

속성

• product name: Human Collagen Type V,
• 생물학적 소스: human
• Quality Level: 100
• 분석: 95% (SDS-PAGE)
• 형태: liquid
• 제조업체/상표: Chemicon^®
• 농도: 1 mg/mL
• 기술: cell culture | mammalian: suitable
• 불순물: <0.5% non-collagen proteins; <1% collagen type III; <2% collagen type I; <2% collagen type IV
• 입력: sample type mesenchymal stem cell(s); sample type induced pluripotent stem cell(s); sample type pancreatic stem cell(s); sample type epithelial cells; sample type neural stem cell(s); sample type hematopoietic stem cell(s); sample type: human embryonic stem cell(s)
• NCBI 수납 번호: NM_000093.3
• UniProt 수납 번호: P20908
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... COL5A1(1289) , COL5A2(1290)

설명

일반 설명

Collagen type V is fibril-forming collagen that is located in the lung, bone, and fetal membranes along with type I collagen. It is minor collagen present in the extracellular matrix.

애플리케이션

Human Collagen Type V has been used to study the interaction of high endothelial venule protein (hevin) with human collagen V by fluorescence polarization (FP) assay and surface plasmon resonance(SPR). It has also been used to incubate the 96 well plate to determine the protein levels in media and cell lysates of fibroblasts.

생화학적/생리학적 작용

Collagen type V acts as a regulator of collagen fibrillogenesis in the cornea and skin dermis. It interacts with matrix collagens and structural proteins thereby contributing to the structural integrity of tissues. Lower levels of type V collagen lead to loss of corneal transparency and Ehler Danlos syndrome.

물리적 형태

Liquid, in 0.1M acetic acid, pH 3.0. No preservatives added.

제조 메모

Purified by serial salt precipitations of a pepsin extraction of human fetal membranes and chromatography on DEAE-cellulose.

저장 및 안정성

Maintain at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze.

기타 정보

Molecular composition: α1(V)]2 α2(V), native triple helix. Purity and retention of the native helical structure were monitored by SDS-PAGE, ORD measurement, and by reaction with anti-collagen type-specific monoclonal antibodies. Product Source: Human placenta, negative for HBsAg and HIV antibodies.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable