ABS492

Anti-RhoGAP1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Rho GTPase-activating protein 1, CDC42 GTPase-activating protein, GTPase-activating protein rhoOGAP, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, p50-RhoGAP, RhoGAP1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, rat, human
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_004299
• UniProt 수납 번호: Q07960
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ARHGAP1(392)

설명

일반 설명

RhoGAP1, also known as Rho GTPase-activating protein 1, CDC42 GTPase-activating protein, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, or p50-RhoGAP, and encoded by the gene ARHGAP1/CDC42GAP/RHOGAP1, is an important GTPase activator for the Rho, Rac, and CDC42 GTP binding proteins, converting them to the putatively inactive GDP-bound state. CDC42 is the preferred substrate for RhoGAP1. Such GTPase-activating proteins or GAPs play an important role in signal transduction pathways regulated by GTP-binding proteins. In addition to acting as down-regulators of GTPases, there is growing evidence that they also act as effector molecules required for downstream signaling. RhoGAP1 is found in multiprotein complexes in the cytoplasm in most cells. Recent research has shown RhoGAP1 to be particularly important during epithelial to mesenchymal morphogenesis and for carcinoma metastasis. Rho-ROCK signaling is essential for cell apical detachment and the generation of motility. RhoGAP1 restricts Rho activation to apical areas and thus helps guide the detachment process and thus is a target of much developmental and cancer research.

면역원

GST-tagged recombinant protein corresponding to human RhoGAP1.

애플리케이션

Anti-RhoGAP1 Antibody is an antibody against RhoGAP1 for use in western blotting & ICC.

Research Category
Signaling

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected RhoGAP1 in 10 µg of A431, NIH/3T3, and PC-12 cell lysates.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected RhoGAP1 in HeLa cells (Schiel, J. A., et al. (2012). Nat Cell Biol. 14(10):1068-1078.).

품질

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected RhoGAP1 in 10 µg of HeLa cell lysate.

표적 설명

~50 kDa observed

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable