ABS2124

Anti-Ferrochelatase

serum, from rabbit

• 동의어(들): Heme synthase, protoheme ferro-lyase
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: serum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, mouse
• 포장: antibody small pack of 25 μL
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_000131
• UniProt 수납 번호: P22830
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FECH(2235)

설명

일반 설명

Ferrochelatase, mitochondrial (UniProt: P22830; also known as EC: 4.99.1.1, Heme synthase, Protoheme ferro-lyase) is encoded by the FECH gene (Gene ID: 2235) in human. Ferrochelatase catalyzes the terminal reaction of the heme biosynthetic pathway in all cells. It catalyzes the insertion of ferrous iron into protoporphyrin IX, yielding heme. In eukaryotic cells, it is located in the inner mitochondrial membrane with its active site facing the mitochondrial matrix. Human ferrochelatase is a homodimer with each subunit composed of five regions: a mitochondrial localization sequence, the N terminal domain, two folded domains, and a C terminal extension. The active pocket of ferrochelatase consists of two hydrophobic "lips" and a hydrophilic interior. The hydrophobic lips face the inner mitochondrial membrane and facilitate the passage of the poorly soluble protoporphyrin IX substrate and the heme product via the membrane. Reduced levels of ferrochelatase activity are observed in patients with protoporphyria. Point-mutations in the FECH gene are reported to be associated with certain cases of erythropoietic protoporphyria.

특이성

This polyclonal antibody detects ferrochelatase in human and murine species. It targets the mature form of ferrochelatase.

면역원

Flag-tagged recombinant fragment corresponding to the mature mitochondrial form containing 369 amino acids (aa 55-423) from human Ferrochelatase.

애플리케이션

Anti-Ferrochelatase, Cat. No. ABS2124, is a rabbit polyclonal antibody that detects Ferrochelatase, mitochondrial. It has been tested for use in Immunoprecipitation and Western Blotting.

Immunoprecipitation Analysis: A representative lot detected Ferrochelatase in differentiated mouse erythroleukemia (MEL) cells (Karr, S.R., et. al. (1988). Biochem J. 254(3):799-803).

Western Blotting Analysis: A representative lot detected Ferrochelatase in differentiated mouse erythroleukemia (MEL) cells and transfected heterologous cells. (Chen, W., et. al. (2010). Blood. 116(4):628-30; Anand, S., et. al. (2009). Clin Cancer Res. 15(10):3333-43; Medlock, A.E., et. al. (2015). PLoS One. 10(8):e0135896).

품질

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Ferrochelatase in 10 µg of K562 cell lysate.

표적 설명

~43 kDa observed; 47.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable