생물학적 소스
rabbit
Quality Level
항체 형태
unpurified
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human
포장
antibody small pack of 25 μL
기술
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
unmodified
유전자 정보
human ... ALAS2(212)
관련 카테고리
일반 설명
5-aminolevulinate synthase, erythroid-specific, mitochondrial (UniProt: P22557; also known as EC: 2.3.1.37, ALAS-E, 5-aminolevulinic acid synthase 2, ALAS2, Delta-ALA synthase 2, Delta-aminolevulinate synthase 2) is encoded by the ALAS2 (also known as ALASE, ASB) gene (Gene ID: 212) in human. Two forms of ALAS have been described in literature; ALAS1 (ALAS-H) and ALAS2 (ALAS-E). ALAS-H is a housekeeping protein, which mediates synthesis of early heme in the mitochondria of most cells. ALAS-E is a mitochondrial matrix homodimeric enzyme that is also involved in catalyzing the first step in heme biosynthetic pathway. Four isoforms of ALAS-E have been described that are generated by alternate splicing. ALAS-E is synthesized with a transit peptide (aa 1-49) that is cleaved to generate a mature form (aa 50-587). Defects in ALAS2 gene are linked to sideroblastic anemia that is characterized by anemia, hypochromic peripheral erythrocytes, systemic iron overload, and by the presence of bone marrow ringed sideroblasts. Defects in ALAS2 gene are also reported in X-linked dominant form of erythropoietic protoporphyria that is characterized by accumulation and increased excretion of porphyrins or their precursors.
특이성
This rabbit polyclonal antibody detects erythroid-specifc 5-aminolevulinate synthase, mitochondrial in human cells.
면역원
Epitope: unknown
Full length recombinant human 5-aminolevulinate synthase.
애플리케이션
Anti-ALAS-E, Cat. No. ABS2121, is a rabbit polyclonal antibody that detects 5-aminolevulinate synthase, erythroid-specific, mitochondrial and has been tested for use in Western Blotting.
Research Category
Signaling
Signaling
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ALAS-E in crude mitochondrial lysate differentiated from DS19 cells.
Western Blotting Analysis: A 1:10,000-25,000 dilution from a representative lot detected ALAS-E in recombinant hALAS isoforms over expressed in E Coli and HEK293T cells transfected with hALAS2 (Courtesy of Dr. Harry A. Dailey at University of Georgia).
Western Blotting Analysis: A 1:10,000-25,000 dilution from a representative lot detected ALAS-E in recombinant hALAS isoforms over expressed in E Coli and HEK293T cells transfected with hALAS2 (Courtesy of Dr. Harry A. Dailey at University of Georgia).
품질
Evaluated by Western Blotting with recombinant hALAS2
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected recombinant ALAS-E.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected recombinant ALAS-E.
표적 설명
~58 and 72 kDa observed; Detects both the precusor and mature forms of human ALAS2. Uncharacterized bands may be observed in some lysate(s).
물리적 형태
Format: Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.
Unpurified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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